Abstract: Genome-wide Brassica campestris short Insertion/Deletion length polymorphisms（InDels）（≤ 5 bp）were detected by aligning whole genome re-sequencing data from two B. campestris accessions，‘R-O-18’and‘L58’，to the reference genome sequence of‘Chiifu-401-42’. In total， we identified 18 479 InDel polymorphsms between‘R-O-18’and‘L58’. A total of 500 InDel polymorphisms（4–5 bp in length）were converted to InDel markers and experimentally validated. Of the selected 500 InDel polymorphisms ，452 yielded a single PCR fragment ， but only 106 showed polymorphism between‘R-O-18’and‘L58’，48 did not amplify a product，346 showed no polymorphism. These 106 InDel markers were used to screen the genotype of RILs developed from the crossing of ‘R-O-18’and‘L58’. A genetic linkage map contained 99 InDel markers was constructed.

Key words: Brassica campestris, insertion/deletions（InDels）, whole genome re-sequencing, Illumina sequencing, genetic linkage map