Abstract: Phenotypic data of cotyledon length（Cl），cotyledon width（Cw），hypocotyl length（Hl），the first pistillate flower bearing node（Fpfn），days to anthesis（Da）in 148 F₉ recombinant inbred lines （RILs）which originated from a narrow-cross between 9110Gt and 9930 were investigated four times in different seasons. The multiple QTL model（MQM）method of software package MapQTL version 4.0 was used to map and analyze QTLs. Nineteen QTLs were detected for 5 traits. These QTLs were mapped on chromosome 1，3，5 and 6，respectively. 17 QTLs（89.5%）were major QTLs which explained more than 10% of the phenotypic variation. Three QTLs were repeatedly detected in different seasons in greenhouse cultivation. Their LOD values varied between 3.28 and 15.25，which explained 6%–37.8% of the phenotypic variation. Based on the whole genome sequence，the genomic regions harboring major QTLs were analyzed using BLAST software.

Key words:

cucumber, seedling-associated traits, quantitative trait locus（QTL）, recombinant inbred lines（RILs）, SSR marker