Abstract: An effective micropropagation system via callus-mediated（indirect）somatic embryo- genesis was established，using in vitro bulblets as explants in Hippeastrum hybridum. The results showed that 2,4-D（0.5 mg · L^-1）and 6-BA（0.5 mg ·  L^-1）had important role in inducing embryogenic callus from in vitro bulblets and in multiplication of callus during sub-cultures. The percentage of friable callus formation was 83.3% in primary culture. The 6-BA（2.5–5.0 mg ·  L^-1）had a notable effect on development of somatic embryos. The rate of callus with more than twenty somatic embryos was about 50% for 50 days. Development of somatic embryos had no difference on the same 6-BA media supplemented with different concentrations of NAA.

Key words: Hippeastrum hybridum, somatic embryogenesis, embryogenic callus