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ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (11): 2175-2181.

• Vegetables • Previous Articles     Next Articles

Generation and Cytological Research of Four Primary Trisomics in Chinese Kale(Brassica alboglabra L. H. Bailey)

LUO  Shuang-Xia, SHEN  二Qiao, ZHANG  Cheng-He, SHEN  Shu-Xing   

  1. (College of Horticulture,Agricultural University of Hebei,Baoding,Heibei 071001,China)
  • Online:2012-11-25 Published:2012-11-25

Abstract: Primary trisomics are important materials for gene assignment. Chinese kale(Brassica alboglabra L. H. Bailey)inbred line‘9501’was used as material,by root tip cell chromosome number examination,34 plants with a extra chromosome(2n + 1 = 19)were selected from the progenies of triploid(2n = 3x = 27)back-crossing with diploid parent(2n = 2x = 18);via propagation in vitro and karyotype analysis,four primary trisomics(Tri-1,Tri-6,Tri-8 and Tri-SAT)were obtained from the 2n + 1 plants. Each trisomic showed some special characters in morphological traits,and mainly formed 8 bivalents and 1 trivalent(8Ⅱ+ 1Ⅲ)at meiotic diakinesis,and most cells got 9 chromosomes in a pole and 10 chromosomes in another pole(9/10)at meiotic anaphaseⅠ. The extra chromosomes of the four trisomics can transmit through female and male gametes,and the transmission rates ranged from 10.15% to 27.63% for female and 4.91% to 11.86% for male.

Key words: Chinese kale, Brassica alboglabra L. H. Bailey, trisomics, karyotype analysis

CLC Number: