Targeted Editing of BoaZDS by CRISPR/Ca9 Technology in Chinese Kale

doi:10.16420/j.issn.0513-353x.2018-0426

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (1): 57-64.doi: 10.16420/j.issn.0513-353x.2018-0426

• Research Papers • Previous Articles Next Articles

Targeted Editing of BoaZDS by CRISPR/Ca9 Technology in Chinese Kale

ZHENG Aihong*，ZHANG Fen*，JIANG Min，YUAN Qiao，JIANG Leiyu，CHEN Qing，TANG Haoru，and SUN Bo**

（College of Horticulture，Sichuan Agricultural University，Chengdu 611130，China）

Online:2019-01-25 Published:2019-01-25

Abstract

Abstract:

The ζ-carotene desaturase（ZDS）gene was selected as the target gene to establish the CRISPR/Cas9 genome editing system in Chinese kale. The target site，near the 5′ end of the coding region of the BoaZDS，was selected，and the CRISPR/Cas9 expression vector was constructed. As a result，a total of 19 transgenic plants were obtained through Agrobacterium-mediated genetic transformation. Sanger sequencing analysis revealed that 13 of them were mutated. The mutation efficiency was 68.42%，and all mutants showed a clear albino phenotype.

Key words: Chinese kale, CRISPR/Cas9, genome editing, ζ-carotene desaturase（ZDS）

CLC Number:

S 635

ZHENG Aihong*，ZHANG Fen*，JIANG Min，YUAN Qiao，JIANG Leiyu，CHEN Qing，TANG Haoru，and SUN Bo**. Targeted Editing of BoaZDS by CRISPR/Ca9 Technology in Chinese Kale[J]. ACTA HORTICULTURAE SINICA, 2019, 46(1): 57-64.

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Targeted Editing of BoaZDS by CRISPR/Ca9 Technology in Chinese Kale

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