Abstract: DNA molecular marker assisted selection of super-male plants was conducted for all-male breeding of asparagus.Males in S₁ population of andromonoecious plants were test-crossed with elite females. Two DNA molecular markers tightly linked to sex determining gene M，Asp1-T7 and Asp1-T7sp，which showed stable and specific amplification，were used for sex identification of individuals in the progeny at seedling stage.Putative‘supermales’（MM）were differentiated from the normal males（Mm） by the presence or absence of‘all-male’population in the progeny. Two out of 14 male plants test crossed were distinguished as putative‘supermales’. The test result of DNA molecular marker-assisted selection was highly consistent with that of the sexual phenotype in field survey.The corresponding combinations originated from the two super-male plants were also identified as all-male combinations.

Key words: Asparagus officinalis, super-male plant, DNA molecular Marker-Assisted Selection, testcross