Abstract: Understand the effect of different combinations of plant growth regulators on bud germination，callus growth induction of stem and leaves，and the regeneration of adventitious buds，by using different tissues of Thymus mandschuricus Ronn.，an endemic ground-cover species of China，as explants. The research results indicated that the axillary buds of Th. mandschuricus can directly germinate in MS + 6-BA 0.5 mg · L-1 + NAA 0.1 mg · L-1 and the germination rate was 74%. The proliferation of axillary buds on MS medium containing 0.5 mg · L-1 6-BA and 0.01 mg · L-1 NAA was achieved to 36.43. The best medium for rooting was 1/2MS + IBA 0.5 mg · L-1. The rooting ratio was 100%. The survival rate reaches 76.7%. Callus can be inducted through the leaf in subculture but can not develop further. The optimizing medium for pedicels explants inducing callus is MS + 6-BA 0.5 mg · L-1 + 2,4-D 1.0 mg · L-1 and inducing multiple buds is MS + 6-BA（0.1–1.0）mg · L-1 + GA3（0.1–0.5）mg · L-1 with the buds induction frequency of 33.3%.

Key words: Thymus mandschuricus, adventitious bud regeneration system, plant growth regulator