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ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (5): 930-938.

• Ornamental Plants • Previous Articles     Next Articles

Preliminary Identification of Leaf Color Mutational Strain from Oxalis triangularis Using RAPD and ISSR Markers

HU Su1,WANG Yong-qing 2,* ,TAO Lian2,FU Yan3,and YANG Qin4   

  1. 1 College of Forestry,Sichuan Agricultural University,Ya’an,Sichuan 625014,China;2 College of Horticulture,Sichuan Agricultural University,Ya’an,Sichuan 625014,China;3 Center for Plant Tissue Culture,Qiandongnan Nationality Professional Technology College,Kaili,Guizhou 556000,China;4 College of Environmental and Life Science,Kaili University,Kaili,Guizhou 556011,China)
  • Received:2010-09-14 Revised:2011-05-06 Online:2011-05-25 Published:2011-05-25
  • Contact: WANG Yong-qing

Abstract: A leaf color mutational strain and the wild type of Oxalis triangularis were identified preliminarily at the molecular level. DNA-pooling method was used in this study to establish the DNA-pools of the wild type and the mutational type,respectively. These DNA-pools were used as templates for RAPD-PCR and ISSR-PCR,respectively. Two specific RAPD primers,i.e.,S76 and S118,and two specific ISSR primers,i.e.,UBC818 and UBC868,were selected from 70 RAPD primers and 100 ISSR primers,respectively,producing 6 specific bands. And these specific bands could identify the two DNA-pools mentioned above. The sequencing result of the absent specific band,S118 700-800,in the mutational type showed that the sequence contained a gene coding for subunit Ⅶ,i.e.,PsaC protein,in photosystemⅠ. The results indicated that compared with the wild type,the genetic material of the mutational strain had changed indeed,and it could maintain stable in the given subcultures in this study.

Key words: Oxalis triangularis A. St.-Hil., leaf color mutation, somaclonal variation, RAPD, ISSR

CLC Number: