Abstract: In this paper，two introgression line（IL）populations respectively derived from the wild species Solanum lycopersicoides LA2951 and S. pennellii LA0716 were used to map the possible loci for drought tolerance during the seedling stage. The results showed that 3 QTLs（qDT-l-3，qDT-l-6 and qDT-l-12）located on chromosome 3，6 and 12 were identified from S. lycopersicoides LA2951 introgression line population and 11 QTLs（qDT-p-1，qDT-p-2a，qDT-p-2b，qDT-p-3，qDT-p-4，qDT-p-5，qDT-p-8，qDT-p-9，qDT-p-10a，qDT-p-10b and qDT-p-12）located on chromosome 1，2，3，4，5，8，9，10 and 12 from S. pennellii LA0716 population. Based on the high density tomato linkage map，QTL located on chromosome 12 might be co-localized from the above two IL populations and QTL qDT-p-9 might be co-localized with a previously identified QTL conferring drought tolerance during seed germination from S. pimpinellifolium LA0722 on chromosome 9.