Abstract: Nineteen primers were used to analysis the genetic relation of 17 macadamia which screened from 100 primers. A total of 369 bands were amplified，of which 249 bands were polymorphic bands（the percentage of polymorphic band，PPB = 67.5%）. Examination of multivariate relationships among accessions using a cluster analysis resulted in the identification of four clusters. All Hawaiian cultivars were included in two sub-clusters within the largest cluster. The results suggesting that the Hawaiian cultivars must have originated from at least two genetically diverse ancestral populations；And the Australian selections，which formed 3 separate clusters，probably represent a different genetic background and selection history in Australia as compared to Hawaiian selections.

Key words: macadamia, genetic relation, ISSR