Genetic Analysis and Mapping of gl-2 Gene in Cucumber（Cucumis sativus L.）

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (09): 1685-1692.

• Vegetables • Previous Articles Next Articles

Genetic Analysis and Mapping of gl-2 Gene in Cucumber（Cucumis sativus L.）

YANG Shuang-juan1，MIAO Han1,*，ZHANG Sheng-ping1，CHENG Zhou-chao1，ZHOU Jian1，DONG Shao-yun1，Todd C. Wehner2，and GU Xing-fang1,**

1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Department of Horticultural Science，North Carolina State University，Raleigh，NC 27695-7609，USA

Received:2011-06-16 Revised:2011-08-23 Online:2011-09-25 Published:2011-09-25
Contact: GU Xing-fang

Abstract

Abstract: Genetic analysis and gene mapping were carried out on gl-2 gene in cucumber（Cucumis sativus L.）using 9110Gt（with trichomes）and NCG-042（glabrous mutant）as experimental materials. The genetic analysis showed having trichomes or not is determined by a single nuclear gene，and the trait of having trichomes（Gl-2）is dominant to the glabrous（gl-2）in cucumber. Bulked segregant analysis（BSA）and simple sequence repeat（SSR）techonology were employed to mapping gl-2 gene of cucmber in F2 population. gl-2 gene was mapped to a linkage group with 11 SSR makers，corresponding to chromosome 2 of cucumber. The flanking markers SSR10522 and SSR132751 were linked to the gl-2 gene with genetic distances of 0.6 and 3.8 cM，respectively. The veracity of SSR10522 and SSR132751 was tested using BC1P2 population，and the accuracy rate for the two markers was 94.4% and 91.6%.

Key words: cucumber, gl-2 gene, genetic analysis, gene mapping, SSR marker

CLC Number:

S 642.2

YANG Shuang-juan;MIAO Han;ZHANG Sheng-ping;CHENG Zhou-chao;ZHOU Jian;DONG Shao-yun;Todd C. Wehner;and GU Xing-fang;. Genetic Analysis and Mapping of gl-2 Gene in Cucumber（Cucumis sativus L.）[J]. ACTA HORTICULTURAE SINICA, 2011, 38(09): 1685-1692.

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Genetic Analysis and Mapping of gl-2 Gene in Cucumber（Cucumis sativus L.）

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