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ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (9): 1499-1506.

• 研究报告 • Previous Articles     Next Articles

Analysis of Castanea mollissima Germplasm Resources by AFLP

LAN Yan-ping,ZHOU Lian-di*,YAO Yan-wu,WANG Shang-de,and LIU Guo-bin   

  1. (Institute of Agricultural Integrated Development,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
  • Received:2010-03-09 Revised:2010-06-25 Online:2010-09-25 Published:2010-09-25
  • Contact: ZHOU Lian-di

Abstract: The genetic variation and genetic relationships of 90 Castanea mollissima cultivars were analyzed by the fluorescent-AFLP technique with 9 primer combinations. Among the 806 bands detected,695 bands were polymorphic,and the high percentage of polymorphic loci(86.23%)revealed a high genetic diversity among the 90 C. mollissima cultivars. The UPGMA phenogram based on genetic identity clustered the 90 C. mollissima cultivars into 2 groups and 14 rows. The 9 pairs of AFLP primer combination used could distinguish all the tested cultivars,the highest ratio of identification is 93% and the lowest is 80%.

Key words: Castanea mollissima, genetic relationship, AFLP, germplasm identification

CLC Number: