Isolation of Parthenocarpy Related ESTs in Parthenocarpic Eggplant Using Suppression Subtraction Hybridization（SSH）

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (12): 1944-1944–1952.

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Isolation of Parthenocarpy Related ESTs in Parthenocarpic Eggplant Using Suppression Subtraction Hybridization（SSH）

ZHOU Ya-jun1，CHEN Yu-hui1，LIU Fu-zhong1,*，ZHANG Ying1,2，and LIAN Yong1

（1Institute of Vegetables & Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2College of Agronomy and Biotechnology，China Agricultural University，Beijing 100193，China）

Received:2010-03-01 Revised:2010-11-01 Online:2010-12-25 Published:2010-12-25
Contact: LIU Fu-zhong

Abstract

Abstract: A forward and a reverse suppression subtraction hybridization（SSH）cDNA library with 472 and 124 positive clones were constructed respectively with cDNA from eggplant（Solanum melongena）parthenocarpic line D-10 ovary as the tester and cDNA from un-parthenocarpic line 03-2 ovary as the driver. After removing repeat and redundancy sequences，384 expressed sequence tags（ESTs）were obtained. Protein homology search in non-redundant protein database revealed that，under E-value less than or equal to 1e-10，121 ESTs were highly homologous with known proteins，and 136 ESTs could be new genes. The results of gene ontology showed that 107，102，119 ESTs were involved in biological process，molecular function and cell component respectively. Five parthenocarpic related ESTs were obtained，including two MADS-box family，one auxin growth promoter protein，one cytochrome P450 NADPH-reductase and one MAPKK family.

Key words: eggplant, parthenocarpy, suppression subtraction hybridization

CLC Number:

S 641.1

ZHOU Ya-jun;CHEN Yu-hui;LIU Fu-zhong;ZHANG Ying;and LIAN Yong. Isolation of Parthenocarpy Related ESTs in Parthenocarpic Eggplant Using Suppression Subtraction Hybridization（SSH）[J]. ACTA HORTICULTURAE SINICA, 2010, 37(12): 1944-1944–1952.

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Isolation of Parthenocarpy Related ESTs in Parthenocarpic Eggplant Using Suppression Subtraction Hybridization（SSH）

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