Colchicines Chemical Induction During Propagaion of Protocorm-like Bodiesof Oncidium in Liquid Culture

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (9): 1385-1389.

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Colchicines Chemical Induction During Propagaion of Protocorm-like Bodiesof Oncidium in Liquid Culture

CUI Guang-rong^1,2;SHANGGUAN Ling-fei¹;ZHANG Zi-xue²;HU Neng-bing²; ZHANG Cong-yu²;HOU Xi-lin^1*

(¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University,Nanjing 210095, China; ² Plant Science School of Anhui Science and Technology University,Fengyang, Anhui 233100, China)

Received:2009-04-30 Revised:2009-06-25 Online:2009-09-25 Published:2009-09-25
Contact: HOU Xi-lin

Abstract

Abstract:

Colchicines were employed to induce polyploid plantlets of Oncidium during ropagation of protocorm-like bodies ( PLB s) in liquid culture. A lot of polyploid plantlets were obtained by this technique.The results showed that the PLBs cultured in the medium contained higher concentration of colchicines got
harmed more severely and the rate of ployploid plantlets was bigger with higher concentration of colchicines or longer time of treatment . The biggest rate of polyploid plantlets regenerated from the PLBs could reach to 46.7%. The mixoploid could be produced under the condition of lower concentrations of colchicines and short time of treatment. The tetraloid plantlets were shorter and stronger and leaves changed broad and thick. The diameter of lower epidermal stomata in the tetraploid was a little bigger than the one in the diploid. The cell nucleus of leaf lower epidermal became bigger and approached to cell wall. The number of chromosomes in root tip cells of the polyploid plantlets were doubled.

Key words: Oncidium, protocorm-like body, polyploid, colchicine, liquid culture, chemical induction

CLC Number:

CUI Guang-rong;SHANGGUAN Ling-fei;ZHANG Zi-xue;HU Neng-bing;ZHANG Cong-yu;HOU Xi-lin. Colchicines Chemical Induction During Propagaion of Protocorm-like Bodiesof Oncidium in Liquid Culture[J]. ACTA HORTICULTURAE SINICA, 2009, 36(9): 1385-1389.

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Colchicines Chemical Induction During Propagaion of Protocorm-like Bodiesof Oncidium in Liquid Culture

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