Abstract: In this article,we studied broccoli karyotype analysis using immature pistil and root tip via squash method. The results indicated that there were more metaphase stage celles in ovary than in stigma and style. Compared with the root tip, the immature ovary possessed more cells which were at metaphase stage; There were no obvious differences in patterns of the morphology chromosome in both materials, we could see satellites in both cells of two materials; No matter which material was used,the karyotype formula both were 2n＝2x=18=8m+10sm(2sat),with 4 metacentric pairs(3，4，7，8)and 5 acrocentric pairs(1，2，5，6, 9) chromosomes, satellites all were observed at the sixth pair of chromosomes. The karyotype all belong to 2A, presented a ultimately symmetry karyotype. In addition, the pistil was more plentiful than root tip when used as preparation of chromosome material. This provided a new method about cytology studies for crucifer crops which have smaller chromosome and satellites.

Key words: broccoli, pistil, root tip, chromosome karyotype