Abstract:

Tetrap loidswere successfully induced from in vitro plantlets of diploid Gerbera jamesoniiBolus‘Cabana’and‘Dalma’(2n=2x=50) by dipping shoot tip s with colchicine. Colchicine at a concentration of 0.02% , 0.05% , 0.10% were tested for 24, 36 and 48 h. The results indicated that the most efficient
condition for inducing tetrap loids was treated with 0110% colchicine for 48 h. The highest mutation rate of‘Cabana’and‘Dalma’was 16% and 10%, respectively. As a whole, gerbrea shoots seemed be not sensibility to colchicines. Mutation budswere screened bymorphological app raisal, stomatal and cytological identification. The chromosome number of part mutants have doubled to 100 (2n=4x=100). As compared with diploid, tetraploid of Gerbera jamesonii Bolus was characterized by larger stoma area, larger flower head,thicker leaves color, leaves tend to round, thicker leaves, larger stem diameter and anthocyanin coloration of peduncle baseswere obviously strong. Finally, over 200 and 300 stable tetrap loids of‘Cabana’and‘Dalma’were respectively abtained.

Key words: Gerbera jamesonii Bolus, polyploidy, ploidy level identification, mutation