Abstract: To research its' salt tolerance mechanism and to get the salt tolerance relative genes of Eustoma grandiflorum, a cDNA library was made by suppression subtractive hybridization technique. The leave mRNA of Eustoma grandiflorum treated with 400 mmol·L^-1 NaCl were tester, and that treated with pure water were driver. From the library, we got 658 unique sequences by sequencing and sequence blasting. 61 of the total sequences are functioning unknown, 535 sequences have no homology and 62 sequences have significance homology to the GO sequence library. The 62 sequences can be classified into 21 function categories. And we finally get the reactive oxygen species scavenging genes: DHAR, GST, TRX, CAT, transcription factor SMT3, and serine/threonine protein kinase gene. It indicated that there are general characters between the salt tolerance genes of Eustoma grandiflorum and model plants, and the most of the sequences can not be explained by the known genes.

Key words: Eustoma grandiflorum, suppression subtractive hybridization, differently expressed genes, salt stress