Physical Localization of 45S rDNA on the Chromosomes of 4 Species of the Genus Lilium

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (6): 859-862.

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Physical Localization of 45S rDNA on the Chromosomes of 4 Species of the Genus Lilium

Zhou Shu-jun^1*,Jaap van Tuyl²,Zang De-kui³,Xia Yi-ping¹,and Li Fang¹

（¹College of Agriculture and Biotechnology, Zhejiang Univeristy, Hangzhou, 310029, China; ²Plant Breeding, Plant Science Group, Wageningen University, P.O. Box 16, Wageningen 6700 AA, The Netherlands; ³College of Forestry, Shandong Agricultural University, Tai'an, Shandong, 271018, China）

Received:2008-01-28 Revised:2008-04-08 Online:2008-06-25 Published:2008-06-25
Contact: Zhou Shu-jun

Abstract

Abstract:

45S rDNA was mapped on the chromosomes of Lilium. longiflorum, L. leichtlinii, L. auratum and L. pardalinum of the genus Lilium using fluorescence in situ hybridization in this paper. The result showed that 45S rDNA were localized near the centromere region in these four species, but the number of the 45S rDNA sites, which chromosomes having the 45S rDNA signal, and the intensity of the 45S rDNA signal somewhat varied in the four species. Three pairs of chromosomes have the 45S rDNA signal in L. longiflorum and L. pardalinum, while four pairs of chromosomes have the 45S rDNA signal in L. leichtlinii and L. auratum. Nevertheless, the two pairs of metacentric chromosomes of L. auratum have the 45S rDNA signal, one pair of metacentric chromosomes of L. leichtlinii have the 45S rDNA signal, but no 45S rDNA signal is on any metacentric chromosome of L. longiflorum and L. pardalinum. Considering the four species belonging to different sections in Lilium, the result supplies some molecular evidence for supporting the Lilium classification.

Key words: Lilium, rDNA, fluorescence in situ hybridization (FISH), karyotype, chromosome

CLC Number:

S 682.2

Zhou Shu-jun;Jaap van Tuyl;Zang De-kui;Xia Yi-ping;and Li Fang. Physical Localization of 45S rDNA on the Chromosomes of 4 Species of the Genus Lilium [J]. ACTA HORTICULTURAE SINICA, 2008, 35(6): 859-862.

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Physical Localization of 45S rDNA on the Chromosomes of 4 Species of the Genus Lilium

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