Study on Preparation of Prototype S-RNase Microarray and Molecule Hybridization Condition

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (4): 475-486.

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Study on Preparation of Prototype S-RNase Microarray and Molecule Hybridization Condition

JIANG Nan^1,2,TAN Xiao-feng¹,CHEN Hong¹,WANG Xiu-li²,ZHANG Lin¹, ZENG Yan-ling¹

(¹The Key Laboratory of Non-Wood Forest Product of State Forestry Administration, Central-South University of Forestry and Technology，Changsha 410004, China; ²Key Laboratory of Green Packaging and Biological Nanotechnology, Hunan University of Technology, Zhuzhou Hunan, 412008, China)

Received:2007-10-19 Revised:2008-03-02 Online:2008-04-25 Published:2008-04-25
Contact: TAN Xiao-feng

Abstract

Abstract:

To explore the application of oligonucleotide microarray in identification of pears S-genotype, some probes for S-RNase detection were designed according to the structure characteristic of self-incompatibility gene, and oligonucleotide microarray was prepared. The PCR products of S-RNase were amplified by Cy3-labeled primer and hybridized with the microarray in order to detect the S-genotype of pears. Optimized reaction conditions were gained by exploration of hybridization temperature and hybridization time. The microarray results of S-genotype of pear was similar with the RFLP and sequenced results. In conclusion:, the oligonucleotide microarray is an effective method in detecting S-genotype of pear. With the future improvement, it could be widely used in the study of self-incompatibility of pear.

Key words: pear, self-incompatibility, S-RNase gene, gene chip, hybridization

JIANG Nan;;TAN Xiao-feng;CHEN Hong;WANG Xiu-li;ZHANG Lin;ZENG Yan-ling . Study on Preparation of Prototype S-RNase Microarray and Molecule Hybridization Condition [J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 475-486.

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Study on Preparation of Prototype S-RNase Microarray and Molecule Hybridization Condition

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