RAPD Analysis of Thirty-eight Cymbidium ensifolium Cultivars

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (2): 289-294.

• 研究简报 • Previous Articles Next Articles

RAPD Analysis of Thirty-eight Cymbidium ensifolium Cultivars

HU Wei¹,HUANG Ru-zhu^1* ,PAN Xiao-hua¹,LI Jin-feng²,and SUN Duan³

( ¹College of Life Sciences, Fujian Normal University, Fuzhou 350108, China; ² Cymbidium ensifolium Garden, Chinese Cymbidium Association, shanghang, Fujian 364200, China;³ The General Inspection Station of Forestry of Fujian Province, Fuzhou 350003, China)

Received:2007-07-12 Revised:2007-12-04 Online:2008-02-25 Published:2008-02-25
Contact: HUANG Ru-zhu

Abstract

Abstract:

DNA from thirty-eight Cymbidium ensifolium cultivars was examined using polymerase chain reaction (PCR) to determine the efficiency of randomly amplified polymorphic DNA (RAPD) markers in genetic diversity and genetic relationship. A total of 116 RAPD markers, 88.79% of which were polymorphic, were produced from 18 10mer arbitrary primers. Genetic distances among the cultivars were estimated based on the amount of band sharing and ranged from 0.0420~0.5385 with an average of 0.2902. Unweighted pair-group method with arithmetic averaging (UPGMA) of genetic distances estimates grouped 'suxin' cultivars and 'caixin' cultivars together with each other, and 'suxin' cultivars diverged from 'caixin' cultivars, thereby agreeing with known traditional classification information. Four cultivars, 'Minxi Yuchen', 'Yuchen Dagong', 'Yuchen' and 'Yinbian Yuchen' could be distinguished from all the rest based only on the S153-650 fragment, and 'Shiliu Luohan' and 'Yuchen' based only on the S38-1 200 fragment. Cultivar 'Ma′er Siji' could be differentiated from all the rest by lack of the S38-800 fragment.

Key words: Cymbidium ensifolium, cultivar, genetic diversity, cluster analysis, RAPD

CLC Number:

S 682.31

HU Wei;HUANG Ru-zhu;PAN Xiao-hua;LI Jin-feng;and SUN Duan. RAPD Analysis of Thirty-eight Cymbidium ensifolium Cultivars [J]. ACTA HORTICULTURAE SINICA, 2008, 35(2): 289-294.

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RAPD Analysis of Thirty-eight Cymbidium ensifolium Cultivars

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