Study on the Genetic Diversity of Some Osmanthus fragrans Cultivars Based on AFLP Markers

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (1): 137-142.

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Study on the Genetic Diversity of Some Osmanthus fragrans Cultivars Based on AFLP Markers

HAN Yuan-ji¹, DONG Mei-fang¹, YUAN Wang-jun², LI Jin¹, HAN Jie¹, and SHANG Fu-de^1*

(¹College of Life Sciences and Institute of Agricultural Biotechnology, Henan University, Kaifeng, Henan 475001, China; ²College of Pharmaceutical Sciences, Henan University, Kaifeng, Henan 475001, China )

Received:2007-08-01 Revised:2007-10-08 Online:2008-01-25 Published:2008-01-25

Abstract

Abstract: 22 primer combinations were used for polymorphic selection, 6 of which could generate reproducible polymorphic fragments and were used to detect the polymorphic sites of 22 Osmanthus fragrans cultivars and 3 species of Osmanthus. A total of 171 bands amplified from 6 primer combinations were selected for AFLP analysis, 104 bands appeared to be polymorphic(60.8%).The data were used to calculate the Nei's genetic distance of the 25 materials and to construct a dendrogram based on UPGMA cluster analysis.The result showed that:It had closely relationship between Osmanthus fragrans cultivars with brunet flower as well as Osmanthus fragrans cultivars with blonde flower,but the relationship between the two groups were remote.From the dendrogram,we knew that some Osmanthus fragrans cultivars of Latifolius Group and Fragrans Group had remotely relationship comparing with Thunbergii Group and Aurantiacus Group.AFLP analysis appeared the taxonomic result was not completely consistent with that of traditional analysis.

Key words: Osmanthus fragrans Lour., Osmanthus, species, AFLP, genetic relationship

CLC Number:

S 685.13

HAN Yuan-ji;DONG Mei-fang;YUAN Wang-jun;LI Jin;HAN Jie;and SHANG Fu-de. Study on the Genetic Diversity of Some Osmanthus fragrans Cultivars Based on AFLP Markers [J]. ACTA HORTICULTURAE SINICA, 2008, 35(1): 137-142.

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Study on the Genetic Diversity of Some Osmanthus fragrans Cultivars Based on AFLP Markers

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