Abstract: In order to establish the technology for separating and purifying total flavonoids from Toona sinensis leaves with macroporous resin, 6 types of macroporous resins were selected to be investigated. The results showed that LSA-10 was the best for separating and purifying total flavonoids in the following conditions: the loaded concentration of total flavones 0.653 mg/mL, pH4.0-5.5, the flow rate 240 mL/h; amount of saturated adsorption for total flavones 440-480mL; 400 mL 50% ethanol as appropriate the eluting reagent, eluting rate 80 mL /h. The purity of total flavonoids purified by LSA-10 was 75.2% when purified four times under the optimal conditions.

Key words: Toona sinensis, Leaf, Total flavonoid, Macroporous resin, Separation, Purification