Abstract: Calli were employed to establish the technical system of tetraploid induction in Anthurium an
draeanum by using colchicine treatment method. The results showed that the induction rate of tetrap loid in
vitro depended on treatmentmethod, concentration of colchicine and duration of treatment. The lower the con
centration of colchicine was, the higher the induction rate was. At the same concentration of colchicine, the
induction rate of tetrap loid using liquid culture was higher than that using solid culture, and the longer the du
ration of treatment was, the higher the induction rate was. The highest induction rate of tetrap loid, 45.5% ,
was obtained from the calli cultured in the liquid medium supp lemented with 0.2 g·L^-1 colchicine for 14 d
on a rotator. The induction rate was not affected by genotype. Tetrap loid of Anthurium and raeanum was char
acterized by larger and thicker leaves and spathes with deeper color and larger size of pollen, anther and
p lant. Its length of stomatal cell on lower ep idermis leaf cellwas longer than that of diploid, while the density
of stomata was less than that of diploid. This indicated that tetrap loids of An thu rium andraeanum could be pro
duced by colchicine treatment and accurately determined by the improved squash method, the length of stoma2
tal cell and the density of stomata.

Key words: Anthurium andraeanum, Polyploid, Stomatal appraisal, Cytological appraisal, Callus