Protoplast Isolation and Plant Regeneration from Soma tic Embryogenic CellSuspension of Celery (Apium graveolens L. )

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (3): 665-670.

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Protoplast Isolation and Plant Regeneration from Soma tic Embryogenic CellSuspension of Celery (Apium graveolens L. )

HAN Qing-xia, SHEN Huo-lin^*;ZHANG Zhen-xian

(Agronomy and Biotechnology College, China Agricultural University, Beijing 100094,China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

Abstract

Abstract: Protop lastswere successfully isolated from embryogenic suspensions incubating in enzyme so
lution containing 3.0% cellulose Onozuka R210, 1.0% macerozyme R210, 0.5% CaCl₂ ·2H₂O, 0.1%
MES and 11% mannitol. The enzyme mixture was shaken (80 r /min) for 6 h at (25 ±2) ℃. The yield and
viability were 25.00 ×10⁶ /g and 83.41%. Purified protop lasts were cultured in mediem 1 /2 MS + 1 mg/L
2, 4-D + 0.5 mg/L KT + 11% mannitol + 500 mg/L CH initially with shallow liquid layers. Two days later,
cell wall was regenerated and simultaneously intiated the first division, through reducing manntol, mini-culli
were observed in 30 days. Shoots regenerated in medium 1 /2MS + 0.25 mg/L KT + 500 mg/L CH.

Key words: Celery, Suspension culture, Protoplast, Regeneration

HAN Qing-xia;SHEN Huo-lin;ZHANG Zhen-xian. Protoplast Isolation and Plant Regeneration from Soma tic Embryogenic CellSuspension of Celery (Apium graveolens L. )[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 665-670.

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Protoplast Isolation and Plant Regeneration from Soma tic Embryogenic CellSuspension of Celery (Apium graveolens L. )

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