Identification and Prokaryotic Expression of Transcription Factor MxMYB1Gene in Malus xiaojinensis

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 833-835.

• 研究报告 • Previous Articles Next Articles

Identification and Prokaryotic Expression of Transcription Factor MxMYB1Gene in Malus xiaojinensis

Cao Dongmei^{1, 2}; Xu Xuefeng¹;Han Zhenhai^1*

(¹Institute of Horticultural Plants, China Agricultural University, Beijing 100094, China; ²Institute of Horticulture, Shanxi Agricultural Academy, Taiyuan, Shanxi 030031, China)

Received:2005-06-25 Revised:2005-09-01 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: MxMYB1 is a MYB transcription factor from Malus xiaojinensis. MxMYB1 contains a highly conserved DNA binding domain and amino acids existed in MYB proteins particularly. Restriction endonuclease analysis, PCR amplifying and sequencing confirmed that construction was correct and had no base mutant and drift. SDS-PAGE of pET-MxMYB1 proteins induced by 1 mmol/L IPTG showed that there was a strengthened protein band in expectant 38 kD protein Marker, but SDS-PAGE of pET-MxMYB1 proteins uninduced by IPTG had not this band. These results will more provide the foundation for purifying and identifying objective
protein.

Key words: Transcription factor MxMYB1, Prokaryotic expression, pET30a-MxMYB1

Cao Dongmei;;Xu Xuefeng;Han Zhenhai. Identification and Prokaryotic Expression of Transcription Factor MxMYB1Gene in Malus xiaojinensis[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 833-835.

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Identification and Prokaryotic Expression of Transcription Factor MxMYB1Gene in Malus xiaojinensis

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