Abstract: The ideal regeneration system of Yunnan wild Roscoea cautleoides by leaves explants was established. In this study, the effects of different place on leaf, plant growth regulators, dark periods, casein hydrolysate, coconutmilk on callus induction and shoot regeneration were investigated. The research results indicated that downside from leaf, dark periods, casein hydrolysate, coconut milk can improve on callus induction. At the same time, dark periods can facilitate shoot regeneration. The app rop riate media for callus induction from leaves explants of Roscoea cautleoides is MS + 6-BA 1.5 mg·L ^{- 1} +NAA 0.1 mg·L ^{- 1} +CH 800 mg·L - 1 + CM 50 mL ·L ^{- 1}. The appropriate media for shoot regeneration is MS + 6-BA 1.5 mg·L ^{- 1} +NAA 0.1 mg·L ^{- 1}. The media for propagation adventitious shoots isMS + 6-BA 0.6 mg·L ^{- 1} +NAA 0.1
mg·L ^{- 1}. The media for regeneration adventitious roots is 1/2MS +NAA 0.8 mg·L ^{- 1}.

Key words: Roscoea cautheoides, Leaf explant, Callus, Regeneration system