SCAR Marker Linked to the Peach/Nectarine in Peach

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (6): 1003-1007.

• 研究论文 • Previous Articles Next Articles

SCAR Marker Linked to the Peach/Nectarine in Peach

Jiang Lijie;Yang Yingjun;Zhang Xiaoming;Li Wensheng;Zhang Kaichun

( ¹ Institute of Forestry and Pomology, Beijing Academ y of Agricultural and Forestry Sciences, Beijing 100093, China; ²College of Agriculture, Henan Science-Technology University, Luoyang 471003, China)

Received:2005-02-03 Revised:2005-06-15 Online:2005-12-25 Published:2005-12-25

Abstract

Abstract: RAPD technique was used in screen molecular marker linked to peach/nectarine character (G / g) in 69 F₁ hybrids resulting from the intraspecific cross between peach cultivar‘J ingyu’and nectarine cultivar‘Flavortop’. The OPP20 primer resulted in a reproducible bright polymorphic band of 2 258 bp present in the individuals of peach character and peach cultivar‘Jingyu’. The polymorphic band was cloned and sequenced. Four primers were designed based on the sequence to transform into SCAR marker. But primer combination BFP94 /BFP95 amp lified the same-sized band in all individuals resulting in a loss of the original RAPD polymorphism. The RAPD markerwas successfully converted into a SCAR markerwith primer combination BFP96 /BFP98, which was designated SCP2022258. The linkage distance is 718 cM between the SCP2022258 and the peach /nectarine character. The SCAR marker will be useful for marker-assisted selection for peach /nectarine character in peach breeding programs.

Key words: Peach, Nectarine, RAPD, SCAR

CLC Number:

S 662.1

Jiang Lijie;Yang Yingjun;Zhang Xiaoming;Li Wensheng;Zhang Kaichun. SCAR Marker Linked to the Peach/Nectarine in Peach[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1003-1007.

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SCAR Marker Linked to the Peach/Nectarine in Peach

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