Full-length cDNA Clone and Sequences Analysis for Unknown Double-stranded RNA Isolated from Radish

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (5): 881-884.

• 研究报告 • Previous Articles Next Articles

Full-length cDNA Clone and Sequences Analysis for Unknown Double-stranded RNA Isolated from Radish

Liu Li1;Chen Jishuang2;Yu Shan3;Wang Chong3

(¹ Faculty of Life Sciences, Huzhou Teachers College, Huzhou 313000, China;² Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China; ³College of Life Sciences, Zhejiang University, Hangzhou 310029, China)

Received:2005-02-15 Revised:2005-05-15 Online:2005-10-25 Published:2005-10-25

Abstract

Abstract: Some characters of unknown dsRNAs isolated from radish were identified. The dsRNAs could be detected from some radish plants and their offspring by dsRNAs analysis approach. The dsRNAswere separated into two fragments in polyacrylamide gel, named dsRNA1 and dsRNA2, respectively with their sizes of
approximately 118 kbp. Full-length cDNA of dsRNA1 was obtained by single primer amplification technique, and its size of 1 866 ntwas determined. RT-PCR also showed that dsRNA1 was of radish plant origin. The results of BLASTP suggested that the biggest putative ORF1 was distantly related to RNA dependent RNA polymerase encoded by some species of Partitivirus. The molecular aspects of dsRNAs, and some biological characters of plants, seem related to radish yellow edge virus (RYEV) previously described.

Key words: Radish, Double-stranded RNA, cDNA cloning, Sequence, Radish yellow edge virus

CLC Number:

S 631.1

Liu Li;Chen Jishuang;Yu Shan;Wang Chong. Full-length cDNA Clone and Sequences Analysis for Unknown Double-stranded RNA Isolated from Radish[J]. ACTA HORTICULTURAE SINICA, 2005, 32(5): 881-884.

[1]	HE Chengyong, ZHAO Xiaoli, XU Tengfei, GAO Dehang, LI Shifang, WANG Hongqing. Genome Sequence Analysis of Strawberry Virus 1 from Shandong Province，China [J]. Acta Horticulturae Sinica, 2023, 50(1): 153-160.
[2]	JIANG Sisi, YUAN Jun, ZHOU Wenjun, NIU Genhua, ZHOU Junqin. Complete Chloroplast Genome Sequence and Characteristics Analysis of Carya illinoinensis [J]. Acta Horticulturae Sinica, 2022, 49(8): 1772-1784.
[3]	JIA Jin, XU Yunlong, ZHOU Jiale, YANG Xiaofen, ZHONG Yonghui, ZENG Jiwu, HONG Ni, WANG Guoping, PENG Shu’ang, DING Fang. Detection and Identification of Huanglongbing Bacteria in‘Cocktail’Grapefruit [J]. Acta Horticulturae Sinica, 2022, 49(3): 590-596.
[4]	DING Yunhua, Budahn Holger, ZHAO Hong, ZHAO Xiuyun. Accurate Identification of Radish Chromosome F in the Backcross Progeny of Brassica rapa and Rape-radish Chromosome F Addition Line [J]. Acta Horticulturae Sinica, 2021, 48(7): 1295-1303.
[5]	HU Wei, LIU Yuxi, ZHAO Qinzheng, CHEN Jinfeng, LOU Qunfeng. Creation and Identification of Cucumis sativus-C. hystrix Alien Addition Lines [J]. Acta Horticulturae Sinica, 2021, 48(7): 1349-1358.
[6]	XIE Dejin, ZHOU Chengcheng, YANG Ke, REN Ke, YANG Deming, CHEN Lingyan, RONG Jundong, ZHENG Yushan. Cloning and Analysis of MoDXS Gene and Its Promoter in Morinda officinalis [J]. Acta Horticulturae Sinica, 2021, 48(3): 577-589.
[7]	BU Fangdi, CHEN Junguang, LIU Zhen, XIANG Benchun, SHEN Mian, CUI Baiming, ZHENG Yinying. Nectarine Stem Pitting-associated Virus and Asian Prunus Virus Found in Prunus persica of Xinjiang [J]. Acta Horticulturae Sinica, 2021, 48(1): 49-59.
[8]	CHEN Dao, ZHANG Jie, WU Zujian, DING Xinlun. Genome Sequence of Strawberry Pallidosis-associated Virus Isolated in China [J]. Acta Horticulturae Sinica, 2021, 48(1): 146-152.
[9]	ZHANG Li*，WANG Qingbiao，and WANG Yanping. Distribution of orf687，a Fertility Restorer Gene for Ogura CMS in Radish [J]. ACTA HORTICULTURAE SINICA, 2020, 47(5): 864-874.
[10]	LIU Jingyu，TENG Ruimin，LI Hui，LIU Hao，and ZHUANG Jing**. Cloning and Expression Analysis under Abiotic Stress of the DHAR Gene in Camellia sinensis [J]. ACTA HORTICULTURAE SINICA, 2020, 47(5): 983-994.
[11]	WEI Qingjiang，MA Zhangzheng，LE Si，LEI Changyu，MA Qiaoli，and GU Qingqing. Identification and Expression Analysis of Sucrose-phosphate Synthase（SPS）Genes in Citrus [J]. ACTA HORTICULTURAE SINICA, 2020, 47(2): 334-344.
[12]	LIU Houyu1,2,3，WU Minfang1，and WEN Xiaopeng 1,*. Cloning and Analysis of LTR Reverse Transcriptase from Chinese Cherry （Prunus pseudocerasus Lindl.） [J]. ACTA HORTICULTURAE SINICA, 2020, 47(2): 355-369.
[13]	ZHENG Yi, ZHANG Hui, WANG Qinmei, GAO Yue, ZHANG Zhihong, and SUN Yuxin. Complete Chloroplast Genome Sequence of Clivia miniata and Its Characteristics [J]. Acta Horticulturae Sinica, 2020, 47(12): 2439-2450.
[14]	QIU Xiaojun, TAN Qunyun, XIAO Qingming, MEI Shiyong, and ZHANG Jifang. Pathogen Identification of Virus Disease and Evaluation for Germplasm Disease Resistance in Radish [J]. Acta Horticulturae Sinica, 2020, 47(10): 1947-1955.
[15]	YANG Guanxian，XU Haifeng，ZHANG Jing，WANG Nan，FANG Hongcheng，JIANG Shenghui，WANG Yicheng，SU Mengyu，and CHEN Xuesen*. Functional Identification of Apple Anthocyanin Regulatory Gene MdMYB111 [J]. ACTA HORTICULTURAE SINICA, 2019, 46(5): 832-840.

Full-length cDNA Clone and Sequences Analysis for Unknown Double-stranded RNA Isolated from Radish

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments