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ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (4): 599-603.

• 研究论文 • Previous Articles     Next Articles

Degradation of Pollen RNA in Japanese Pear Style in Vitro

Xu Yiliu;Zhang Shaoling   

  1. (College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:2004-10-15 Revised:2005-03-28 Online:2005-08-25 Published:2005-08-25

Abstract: The RNAs were isolated from styles that were self- and cross-pollinated with 32 P-labelled pollens in vivo. Than radioactivity of pollinated style RNA was determined with a liquid scintillation counter, and agarose gel electrophoresis was used to test the degradation of RNA from pollinated styles. Results were
that the content of pollen 32 P-RNA in self-pollinated style RNA is 40.7% - 25.3% that of pollen 32 P-RNA in cross-pollinated style RNA after pollination 12 - 48 h, and that pollen intact RNA in self-pollinated style was specifically decreased. This indicated that pollen RNA in self-pollinated style was specifically degraded.

Key words: Pear, Style, Pollen, RNA, Degradation, Self2incompatibility

CLC Number: