Abstract: In vitro morphogenesis pathways and anatomic differences of eight kinds of calli in Anthurium andraeanum ‘Jolanba’at different culture stages or on the culture media supplemented with different cytokinins were researched. The results showed that the main pathwaywas indirect organgenesis, and adventitious shoot primordium derived from the subsurface layers of callus. Somatic embryogenesiswas also found both inside and on the surface of callus. Eight kinds of calli differed in types of nodule and number and distribution
of meristematic cells. Number and distributing of meristematic cells was well related to shoot differentiation.Callus with more meristematic cells or outer-distributed meristematic cells could differentiate more shoots.Thidiazuron (TDZ) was showed a good effect on the induction ofmeristematic cells and somatic embryo. Nicking and making incision on callus surfaceswas helpful for shoot differentiation.

Key words: Anthurium andraeanum, Callus, Anatomic structure, Morphogenesis pathway, Nodule