Abstract:

In order to investigate the in vitro morphologic pathway of meristematic nodules from
adventitious root of Pulsatilla koreana Nakai，young leaves were used as materials to induce adventitious
root，then meristematic nodules were induced using adventitious roots. Top of the early stage of
meristematic nodule consisted of 3–4 meristematic cells，small and deeply stained small cells were widely
distributed at epidermal layers and cortex. Two kinds of tissue type were observed at paraffin section，one
type tissue with meristematic cell，vascular component，parenchymatous cells，leaf primordia and shoot
apex. The other type tissue consisted of parenchymatous cells were surrounded by regularly arranged
epidermal layers. Globular，heart，oval stage somatic embryo were widely distributed on the surface of
tissue. Finally，shoot organogenesis and somatic embryogenesis were developed from meristematic nodules.

Key words: Pulsatilla koreana, adventitious root, meristematic nodule, organogenesis, somatic
embryogenesis, histological examination