Plant Regeneration from Immature Embryos of Ginkgo biloba

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (1): 105-107.

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Plant Regeneration from Immature Embryos of Ginkgo biloba

Guo Changlu;Chen Ligeng;He Xinhua;Syed Asgar Tori

(¹ Key Laboratory of Horticultural Plant Growth Development and Biotechnology, China Ministry of Agriculture, Zhejiang University, Hangzhou 310029, China; ² School of the Ocean, Harbin Institute of Technology, Weihai 264209, China; ³AgriculturalCollege of Guangxi University, Nanning 530005, China)

Received:2004-02-02 Revised:2004-04-13 Online:2005-02-25 Published:2005-02-25

Abstract

Abstract: Factors influencing the induction of callus and somatic embryogenesis were studied with immature embryos of Ginkgo biloba. Highest rate (53.6% ) of somatic embryo genesis was obtained on MK medium containing NAA 1.0 mg·L ^{- 1} +BA 1.0 mg·L ^{- 1}. Maximum number of embryoids observed on callus was 38 with 3 mm plus embryos and 34.5% embryoids could be developed into plants. Darkness is not suitable for somatic embryogenesis. The growth and development of somatic embryos were promoted by adding 10% CM into the medium.

Key words: Ginkgo biloba, Embryo culture, Somatic embryogenesis

CLC Number:

s664.3

Guo Changlu;Chen Ligeng;He Xinhua;Syed Asgar Tori. Plant Regeneration from Immature Embryos of Ginkgo biloba[J]. ACTA HORTICULTURAE SINICA, 2005, 32(1): 105-107.

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Plant Regeneration from Immature Embryos of Ginkgo biloba

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