In Vitro Morphogenesis of Begonia tuberhybrida

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (5): 694-696.

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In Vitro Morphogenesis of Begonia tuberhybrida

Liu Fuping;Su Minghua;Zhang Ning;Lin Qinghong

( Fujian Institute of Subtropical Botany , Xiamen 361006 , China)

Received:2003-12-17 Revised:2004-07-23 Online:2004-10-25 Published:2004-10-25

Abstract

Abstract: Organogenesis and somatic embryogenesis of Begonia tuberhybrida could carry out on the same culture conditions. Somatic embryos occurred from explant directly or indirectly and originated f rom single-cell or multi-cell. At higher induce temperature (28 ℃) , subculturing regenerated young leaf could establish high efficient regenerate system. Through subculturing cluster of buds , a large amount of callus formed , and the callus subcultured for half a year lost almostly differential ability. Observation showed callus was habituated by growth substance and there were wide-ranging variation in the number of chromosome in cells of the callus.

Key words: Begonia tuberhybrida, Somatic embryogenesis, Regenerate system, Subculture callus

CLC Number:

S 68

Liu Fuping;Su Minghua;Zhang Ning;Lin Qinghong. In Vitro Morphogenesis of Begonia tuberhybrida[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 694-696.

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In Vitro Morphogenesis of Begonia tuberhybrida

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