Abstract: An efficient and simple method for shoots regeneration from in vitro leaves of ‘Gisela 5’ and ‘Gisela 6’ (Pruns cerasus×P．canescens)was developed．Factors influencing in vitro leaf regeneration were studied．The ability to regenerate shoot from in vitro leaves of‘Gisela 6’ was much greater than that of ‘Gisela 5’：Optimal results were obtained by culturing leaves adaxial side down on WPM medium containing BA 5 mg/L+IBA 0.5 mg/L and BA 7 mg/L+IBA 0.3 mg/L respectively in the dark for 15 days．Shoot regeneration rate of ‘Gisela 6’ reached up to 10o％ and that of ‘Gisela 5’ was 75.1% ．The regeneration shoot was easy to rooting and to transplant to the field．

Key words: Cherry, Rootstock, Dwarf, In vitro leaf, Regeneration, Tissue culture