Regeneration of Dodecapioid Plants from in Vitro Leave of 'Luotian Tianshi' Persimmon Treated with Colchicine

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (03): 325-327.

• 研究报告 • Previous Articles Next Articles

Regeneration of Dodecapioid Plants from in Vitro Leave of 'Luotian Tianshi' Persimmon Treated with Colchicine

Gu Xiaofeng¹;Luo Zhengrong²

( ¹College of Horticulture and Forestry，Huazhong Agriculture University,Wuhan 430070, China; ²Department of Horticulture Hubei Institute for Nationalities，Enshi 445000，China)

Received:2002-06-05 Revised:2002-09-19 Online:2003-06-25 Published:2003-06-25

Abstract

Abstract: A study of inducing polyploidy by treating ‘Luotian Tianshi’persimmon in vitro leaflet explants with colchicine solution was perfonned．The results indieated that mutated planflets can be induced by treating the leaflet in 0.3％ colchicines solution for 4—6 d．Stomata of the mutated planflets were significantly larger than those of the normal planflets．but the density of stomata were reduced signmcantly．The chromosome number of root—tip cell of mutated plantlets was 2n=12x=180， and nuclear DNA content of the mutated planflets were twice as much as normal hexaploid plantlets．These results suggest that the ploidy of mutated planflets was autododecaploid．

Key words: Persimmon, Colchicine, Dodecaploid, Regeneration

CLC Number:

S 665.2

Gu Xiaofeng;Luo Zhengrong. Regeneration of Dodecapioid Plants from in Vitro Leave of 'Luotian Tianshi' Persimmon Treated with Colchicine[J]. ACTA HORTICULTURAE SINICA, 2003, 30(03): 325-327.

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Regeneration of Dodecapioid Plants from in Vitro Leave of 'Luotian Tianshi' Persimmon Treated with Colchicine

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