Studies on Chromosome Preparations Using Plant Tendril as Source Tissue

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (4): 378-380.

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Studies on Chromosome Preparations Using Plant Tendril as Source Tissue

Chen Jinfeng and Qian Chuntao

(State Key Laboratory of Crop Genetics and Germplasm Enhancement , College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

Abstract

Abstract: Plant tendrils of several horticulture species were used as tissue source to research the procedure of making-slides for chromosome preparation. The condition required by tendrils was more stringent than that required
by root tips in pretreatment and hydrolyzation. Through the developed procedure , chromosome number of the new species Cucumis ×hytivus Chen & Kirbride was first cytogenetically confirmed to be 2n = 38. When root tips
cannot be obtained easily , this technique is an optimal alternate method for chromosome visualization in tendril-producing species , and especially valuable for some rare genetic germplasm.

Key words: Tendril, Chromosome

CLC Number:

S 60

Chen Jinfeng and Qian Chuntao. Studies on Chromosome Preparations Using Plant Tendril as Source Tissue[J]. ACTA HORTICULTURAE SINICA, 2002, 29(4): 378-380.

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Studies on Chromosome Preparations Using Plant Tendril as Source Tissue

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