Abstract: Effective plant regeneration was obtained on MS medium supplemented with NAA 1 mg/ L and BA 2 mg/ L , using leaves of basal bulb as explants in Amaryllis vittata. The highest callus induction rate was 93. 3 % and shoot induction rate was 90. 0 %. The effect of NAA on morphogenesis was better than 2 ,42D , and that of BA was better than KT. Embryoid was induced after 4 times of subculture of callus. The regenerated shoots and embryoids were rooted on MS mediumwith no hormones. Plantlets with roots were then transplanted into vermiculite sprayed with MS mineral elements once every two days with the survival rate of more than 98 % , or the regenerated shoots with no roots were planted in sterile vermiculite whose survival rate reached more than 85 % in order to save culture time.

Key words: Amaryllis vittata, Bulb, In vitro culture, Morphogenesis, Embryoid