Alginate Oligosaccharides Induced CsWRKY46 to Regulate Cucumber Response to Cold Stress and Transcriptome Analysis

doi:10.16420/j.issn.0513-353x.2025-0354

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (5): 1285-1300.doi: 10.16420/j.issn.0513-353x.2025-0354

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Alginate Oligosaccharides Induced CsWRKY46 to Regulate Cucumber Response to Cold Stress and Transcriptome Analysis

ZHU Fangyi, SU Hongling, GU Linghe, ZHANG Chong, PANG Hongbo, TONG Deli^*(), ZHANG Ying^*()

College of Life Science， Shenyang Normal University， Shenyang 110034， China

Received:2025-06-10 Revised:2026-02-12 Online:2026-05-25 Published:2026-05-26
Contact: TONG Deli, ZHANG Ying

Abstract

Abstract:

This paper aims to explore the molecular mechanism of CsWRKY46 gene induced by alginate oligosaccharides in cucumber response to cold stress. In this study，cucumber CU2（wild type） and CsWRKY46 gene knockout type were used as materials，and 0.8% alginate oligosaccharides was sprayed before 4 ℃ cold stress treatment. The effects of alginate oligosaccharides on antioxidant enzyme activity，malondialdehyde content and osmotic adjustment substance content of cucumber seedlings were analyzed，and transcriptome sequencing analysis was carried out. The results showed that the pretreatment of alginate oligosaccharide significantly improved the antioxidant capacity and cold tolerance of cucumber seedlings under cold stress. Transcriptome sequencing results showed that the differentially expressed genes induced by alginate oligosaccharides were mainly enriched in plant hormone signal transduction and protein processing，among which the gene expression of abscisic acid and brassinolide signaling pathways was significantly up-regulated. These results suggest that alginate oligosaccharides may enhance the tolerance of cucumber to cold stress by regulating CsWRKY46 gene and its downstream signaling pathway.

Key words: cucumber, WRKY, cold stress, alginate oligosaccharides, transcriptome sequencing

ZHU Fangyi, SU Hongling, GU Linghe, ZHANG Chong, PANG Hongbo, TONG Deli, ZHANG Ying. Alginate Oligosaccharides Induced CsWRKY46 to Regulate Cucumber Response to Cold Stress and Transcriptome Analysis[J]. Acta Horticulturae Sinica, 2026, 53(5): 1285-1300.

Figures/Tables 10

References 38

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受害级别 Injury level	总体症状描述 Overall symptom description	具体叶片受害情况 Specific leaf damage situation
S0	无受害症状 No symptoms of victimization	所有叶片均无受害症状。 All leaves had no damage symptoms.
S1	轻微受害 Minor victimization	叶片稍皱缩；下数第1或第2叶叶缘发黄或略失水；第3叶和新叶（心叶）无受害症状。 Leaves slightly shr；the lower 1st or 2nd leaf margin is yellow or slightly dehydrated；the third leaf and new leaf（heart leaf）had no symptoms.
S2	中度受害 Moderate victimization	叶片皱缩；下数第1叶和第2叶叶缘严重失水；第3叶叶缘发黄或略为失水；心叶无明显受害症状。 Leaf shrinkage；the first leaf and the second leaf edge serious water loss；the third leaf margin is yellow or slightly dehydrated；there were no obvious symptoms of heart leaf injury.
S3	较重受害 Heavy victimization	下数第1叶和第2叶中部出现脱水斑；第3叶叶缘严重失水；心叶轻微失水。 Dehydration spots appeared in the middle of the first and second leaves. The third leaf margin is seriously dehydrated；heart leaves slightly dehydrated.
S4	严重受害（部分可恢复） Seriously victimized（partially recoverable）	下数第1叶和第2叶中部脱水斑连接成片，叶片萎蔫；第3叶中部开始显现脱水斑；心叶失水较明显；但幼苗置于常温下，心叶尚能恢复。 The dehydration spots in the middle of the first and second leaves were connected into pieces，and the leaves wilted. Dehydration spots began to appear in the middle of the third leaf；the water loss of the heart leaf is obvious；however，when the seedlings were placed at room temperature，the heart leaves could still recover.
S5	极度严重受害（不可恢复） Extremely serious victimization（unrecoverable）	所有叶片严重失水萎蔫；幼苗即使再置于常温下也不能恢复。 All leaves were severely dehydrated and wilted；the seedlings could not recover even under normal temperature.

受害级别 Injury level	总体症状描述 Overall symptom description	具体叶片受害情况 Specific leaf damage situation
S0	无受害症状 No symptoms of victimization	所有叶片均无受害症状。 All leaves had no damage symptoms.
S1	轻微受害 Minor victimization	叶片稍皱缩；下数第1或第2叶叶缘发黄或略失水；第3叶和新叶（心叶）无受害症状。 Leaves slightly shr；the lower 1st or 2nd leaf margin is yellow or slightly dehydrated；the third leaf and new leaf（heart leaf）had no symptoms.
S2	中度受害 Moderate victimization	叶片皱缩；下数第1叶和第2叶叶缘严重失水；第3叶叶缘发黄或略为失水；心叶无明显受害症状。 Leaf shrinkage；the first leaf and the second leaf edge serious water loss；the third leaf margin is yellow or slightly dehydrated；there were no obvious symptoms of heart leaf injury.
S3	较重受害 Heavy victimization	下数第1叶和第2叶中部出现脱水斑；第3叶叶缘严重失水；心叶轻微失水。 Dehydration spots appeared in the middle of the first and second leaves. The third leaf margin is seriously dehydrated；heart leaves slightly dehydrated.
S4	严重受害（部分可恢复） Seriously victimized（partially recoverable）	下数第1叶和第2叶中部脱水斑连接成片，叶片萎蔫；第3叶中部开始显现脱水斑；心叶失水较明显；但幼苗置于常温下，心叶尚能恢复。 The dehydration spots in the middle of the first and second leaves were connected into pieces，and the leaves wilted. Dehydration spots began to appear in the middle of the third leaf；the water loss of the heart leaf is obvious；however，when the seedlings were placed at room temperature，the heart leaves could still recover.
S5	极度严重受害（不可恢复） Extremely serious victimization（unrecoverable）	所有叶片严重失水萎蔫；幼苗即使再置于常温下也不能恢复。 All leaves were severely dehydrated and wilted；the seedlings could not recover even under normal temperature.

处理Treatment	野生型 Wild type	CsWRKY46-CR1	CsWRKY46-CR2
T1	0.25 b ± 0.0038	0.35 a ± 0.0077	0.40 a ± 0.0067
T2	0.20 c ± 0.0067	0.28 b ± 0.0067	0.32 b ± 0.0067

处理Treatment	野生型 Wild type	CsWRKY46-CR1	CsWRKY46-CR2
T1	0.25 b ± 0.0038	0.35 a ± 0.0077	0.40 a ± 0.0067
T2	0.20 c ± 0.0067	0.28 b ± 0.0067	0.32 b ± 0.0067

Alginate Oligosaccharides Induced CsWRKY46 to Regulate Cucumber Response to Cold Stress and Transcriptome Analysis

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