Preliminary Transcriptome Analysis of Folate Synthesis and Metabolism in Cabbage

doi:10.16420/j.issn.0513-353x.2025-0045

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (5): 1301-1316.doi: 10.16420/j.issn.0513-353x.2025-0045

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Preliminary Transcriptome Analysis of Folate Synthesis and Metabolism in Cabbage

ZHANG Yue¹^,², FENG Yiliao¹, WANG Bei², REN Wenjing¹, JIANG Chunyu¹, ZHAO Xinyu¹, WANG Caihong¹, YANG Limei¹, ZHUANG Mu¹, LÜ Honghao¹, WANG Yong¹, ZHANG Yangyong¹^,^*(), JI Jialei¹^,³^,^*()

¹ State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
² College of Horticulture，Shenyang Agricultural University，Shenyang 110161，China
³ China Vegetable Seed Technology Co., Ltd.（Chongqing），Chongqing 402560，China

Received:2025-04-13 Revised:2025-04-27 Online:2025-05-23 Published:2025-05-21
Contact: ZHANG Yangyong, JI Jialei

Abstract

Abstract:

A targeted metabolomic analysis was conducted on the folate composition and content of 127 cabbage inbred lines. The main folate components in cabbage were identified as 5-methyl- tetrahydrofolate（60%-80% of the total）and 5-formyl-tetrahydrofolate（15%-25% of the total）. Early-maturing，round-headed high-folate inbred line Q428 and low-folate inbred line Q372 were selected for further study. Comparative transcriptomic analysis of Q428 and Q372 identified 10 305 differentially expressed genes，with 5 178 genes upregulated and 5 127 genes downregulated in Q428. Gene Ontology analysis indicated that the differentially expressed genes were associated with processes such as photosynthesis，light harvesting，protein-chromophore interactions，and chlorophyll binding. KEGG pathway analysis revealed that these genes were primarily involved in carbon metabolism，amino acid biosynthesis，and starch and sucrose metabolism.Through a collinearity analysis with 24 Arabidopsis folate biosynthesis genes，a total of 57 folate-related genes were identified in cabbage，of which 12 key genes showed significant differential expression between Q428 and Q372. Compared to Q372，the expression levels of BoGCHI，genes associated with the GDC complex（BoGCSH，BoGCSL，BoGCSP，BoGCST），BoMTHFR，and BoSHMT1a were significantly upregulated in Q428，while the expression of BoGGH was significantly downregulated. The differential expression of these genes is preliminarily hypothesized to be a key factor affecting the folate content in cabbage，and these genes could serve as molecular targets for manipulating folate content in cabbage through biotechnological breeding techniques.

Key words: cabbage, folate, transcriptome, metabolome

ZHANG Yue, FENG Yiliao, WANG Bei, REN Wenjing, JIANG Chunyu, ZHAO Xinyu, WANG Caihong, YANG Limei, ZHUANG Mu, LÜ Honghao, WANG Yong, ZHANG Yangyong, JI Jialei. Preliminary Transcriptome Analysis of Folate Synthesis and Metabolism in Cabbage[J]. Acta Horticulturae Sinica, 2025, 52(5): 1301-1316.

Figures/Tables 13

References 25

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样品Sample	双端序列总数Paired-end reads counts	总碱基数Clean bases	GC含量/% GC Content	碱基质量值/% ≥ 30
Q372-1	20 186 702	6 043 240 774	46.57	94.02
Q372-2	21 382 384	6 402 029 466	46.70	94.50
Q372-3	19 720 185	5 902 457 860	46.32	94.24
Q428-1	21 147 787	6 332 933 914	47.79	93.93
Q428-2	23 999 831	7 185 985 424	47.75	94.32
Q428-3	19 953 645	5 975 348 582	47.70	94.16

样品Sample	双端序列总数Paired-end reads counts	总碱基数Clean bases	GC含量/% GC Content	碱基质量值/% ≥ 30
Q372-1	20 186 702	6 043 240 774	46.57	94.02
Q372-2	21 382 384	6 402 029 466	46.70	94.50
Q372-3	19 720 185	5 902 457 860	46.32	94.24
Q428-1	21 147 787	6 332 933 914	47.79	93.93
Q428-2	23 999 831	7 185 985 424	47.75	94.32
Q428-3	19 953 645	5 975 348 582	47.70	94.16

功能途径 Function pathway	基因 Gene	蛋白缩写 Protein abbreviation	拟南芥ID Arabidopsis thaliana ID	甘蓝ID Brassica oleracea ID	相似度/% Similarity	E值 E-value
pABA合成 pABA synthesis	ADCL	ADCL	AT1G50090.1	BolC02g022020.2J	80.548	0
				BolC03g082990.2J	85.955	0
				BolC06g004630.2J	89.045	0
	ADCS	ADCS	AT2G28880.1	BolC04g021330.2J	84.832	0
	ADCS	ADCS	AT2G28880.1	BolC04g055450.2J	81.031	0
喋啶合成 Pteridine synthesis	NUDT	DPP	AT1G68760.1	BolC02g025120.2J	82.270	9.78E-87
	DHNA	DHNA	AT3G11750.1	BolC01g050940.2J	87.500	3.6E-74
	GCHI	GCHI	AT3G07270.1	BolC05g058460.2J	82.303	0
	GCHI	GCHI	AT3G07270.1	BolC01g053860.2J	81.023	0
组装加尾 Assembly and taliling	DHTS/DHFR	DHFR	AT2G16370.1	BolC03g051860.2J	89.382	0
				BolC01g004210.2J	85.246	0
				BolC07g057740.2J	88.690	5.2E-103
	DHFS	DHFS	AT5G41480.1	BolC07g028910.2J	86.090	0
	HPPK/DHPS	HPPK/DHPS	AT4G30000.2	BolC01g009090.2J	85.257	0
分布转运 Distribution and transportation	FPGS	FPGS	AT3G10160.1	BolC01g052470.2J	82.083	0
分布转运 Distribution and transportation	GGH	GGH	AT1G78660.1	BolC02g034750.2J	85.893	0
降解回补Degradation and replenishment	PTAR	PTAR	AT1G10310.1	BolC08g021880.2J	92.181	5.2E-168
C1代谢 C1 metabolism	5FCL	5FCL	AT5G13050.1	BolC09g060020.2J	85.816	4.2E-165
	5FCL	5FCL	AT5G13050.1	BolC02g005400.2J	79.196	0
	5FCLL	5FCLL	AT1G76730.1	BolC06g030250.2J	86.517	0
	DHC	DHC	AT2G38660.1	BolC04g063230.2J	88.385	0
	FTHS	FTHS	AT1G31220.1	BolC05g029350.2J	83.390	1.4E-172
	GCSH	GCSH	AT1G32470.1	BolC08g011080.2J	94.012	3.1E-105
				BolC05g037930.2J	95.210	2.9E-107
				BolC05g030770.2J	93.413	6.4E-105
				BolC04g013540.2J	91.018	8E-101
	GCSL	GCSL	AT1G48030.1	BolC06g002320.2J	94.488	0
				BolC05g049030.2J	95.079	0
				BolC05g049060.2J	91.262	1.94E-57
	GCSP	GCSP	AT4G33010.1	BolC01g005590.2J	90.172	0
	GCST	GCST	AT1G11860.1	BolC08g055130.2J	97.066	0
	GCST	GCST	AT1G11860.1	BolC08g023150.2J	95.854	0
	MS	MS	AT5G17920.1	BolC03g009250.2J	97.781	0
				BolC09g055230.2J	97.520	0
				BolC02g008490.2J	97.258	0
				BolC03g036730.2J	95.953	0
				BolC05g061690.2J	95.181	0
				BolC03g011020.2J	91.054	0
				BolC02g025710.2J	95.200	8.66E-81
	MTHFR	MTHR	AT2G44160.1	BolC04g005400.2J	94.454	0
				BolC04g066800.2J	91.611	0
				BolC06g025320.2J	96.121	0
				BolC08g043230.2J	94.098	0
				BolC02g000140.2J	80.519	3.77E-35
	PURU	10-FDF	AT4G17360.1	BolC01g023770.2J	93.617	0
				BolC07g030320.2J	93.272	0
				BolC07g042860.2J	79.688	9.51E-27
				BolC07g030040.2J	82.759	9.29E-28
	SHMT1a	SHMTla	AT4G37930.1	BolC03g073550.2J	98.263	0
				BolC01g001230.2J	97.297	0
				BolC06g042720.2J	91.573	0
				BolC06g033950.2J	89.513	0
				BolC07g059720.2J	81.176	1.01E-35
	SHMT3	SHMT3	AT4G32520.1	BolC01g006620.2J	91.018	0
				BolC03g081120.2J	88.868	0
				BolC07g056500.2J	96.970	1.9E-14
				BolC07g056470.2J	96.970	1.9E-14

功能途径 Function pathway	基因 Gene	蛋白缩写 Protein abbreviation	拟南芥ID Arabidopsis thaliana ID	甘蓝ID Brassica oleracea ID	相似度/% Similarity	E值 E-value
pABA合成 pABA synthesis	ADCL	ADCL	AT1G50090.1	BolC02g022020.2J	80.548	0
				BolC03g082990.2J	85.955	0
				BolC06g004630.2J	89.045	0
	ADCS	ADCS	AT2G28880.1	BolC04g021330.2J	84.832	0
	ADCS	ADCS	AT2G28880.1	BolC04g055450.2J	81.031	0
喋啶合成 Pteridine synthesis	NUDT	DPP	AT1G68760.1	BolC02g025120.2J	82.270	9.78E-87
	DHNA	DHNA	AT3G11750.1	BolC01g050940.2J	87.500	3.6E-74
	GCHI	GCHI	AT3G07270.1	BolC05g058460.2J	82.303	0
	GCHI	GCHI	AT3G07270.1	BolC01g053860.2J	81.023	0
组装加尾 Assembly and taliling	DHTS/DHFR	DHFR	AT2G16370.1	BolC03g051860.2J	89.382	0
				BolC01g004210.2J	85.246	0
				BolC07g057740.2J	88.690	5.2E-103
	DHFS	DHFS	AT5G41480.1	BolC07g028910.2J	86.090	0
	HPPK/DHPS	HPPK/DHPS	AT4G30000.2	BolC01g009090.2J	85.257	0
分布转运 Distribution and transportation	FPGS	FPGS	AT3G10160.1	BolC01g052470.2J	82.083	0
分布转运 Distribution and transportation	GGH	GGH	AT1G78660.1	BolC02g034750.2J	85.893	0
降解回补Degradation and replenishment	PTAR	PTAR	AT1G10310.1	BolC08g021880.2J	92.181	5.2E-168
C1代谢 C1 metabolism	5FCL	5FCL	AT5G13050.1	BolC09g060020.2J	85.816	4.2E-165
	5FCL	5FCL	AT5G13050.1	BolC02g005400.2J	79.196	0
	5FCLL	5FCLL	AT1G76730.1	BolC06g030250.2J	86.517	0
	DHC	DHC	AT2G38660.1	BolC04g063230.2J	88.385	0
	FTHS	FTHS	AT1G31220.1	BolC05g029350.2J	83.390	1.4E-172
	GCSH	GCSH	AT1G32470.1	BolC08g011080.2J	94.012	3.1E-105
				BolC05g037930.2J	95.210	2.9E-107
				BolC05g030770.2J	93.413	6.4E-105
				BolC04g013540.2J	91.018	8E-101
	GCSL	GCSL	AT1G48030.1	BolC06g002320.2J	94.488	0
				BolC05g049030.2J	95.079	0
				BolC05g049060.2J	91.262	1.94E-57
	GCSP	GCSP	AT4G33010.1	BolC01g005590.2J	90.172	0
	GCST	GCST	AT1G11860.1	BolC08g055130.2J	97.066	0
	GCST	GCST	AT1G11860.1	BolC08g023150.2J	95.854	0
	MS	MS	AT5G17920.1	BolC03g009250.2J	97.781	0
				BolC09g055230.2J	97.520	0
				BolC02g008490.2J	97.258	0
				BolC03g036730.2J	95.953	0
				BolC05g061690.2J	95.181	0
				BolC03g011020.2J	91.054	0
				BolC02g025710.2J	95.200	8.66E-81
	MTHFR	MTHR	AT2G44160.1	BolC04g005400.2J	94.454	0
				BolC04g066800.2J	91.611	0
				BolC06g025320.2J	96.121	0
				BolC08g043230.2J	94.098	0
				BolC02g000140.2J	80.519	3.77E-35
	PURU	10-FDF	AT4G17360.1	BolC01g023770.2J	93.617	0
				BolC07g030320.2J	93.272	0
				BolC07g042860.2J	79.688	9.51E-27
				BolC07g030040.2J	82.759	9.29E-28
	SHMT1a	SHMTla	AT4G37930.1	BolC03g073550.2J	98.263	0
				BolC01g001230.2J	97.297	0
				BolC06g042720.2J	91.573	0
				BolC06g033950.2J	89.513	0
				BolC07g059720.2J	81.176	1.01E-35
	SHMT3	SHMT3	AT4G32520.1	BolC01g006620.2J	91.018	0
				BolC03g081120.2J	88.868	0
				BolC07g056500.2J	96.970	1.9E-14
				BolC07g056470.2J	96.970	1.9E-14

Preliminary Transcriptome Analysis of Folate Synthesis and Metabolism in Cabbage

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