Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (8): 1792-1802.doi: 10.16420/j.issn.0513-353x.2023-0385
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
ZHAO Jingyi, WU Xiaoxu, HU Yunjie, GAI Shuting, ZHU Zhihao, QIN Lei*(), WANG Yong*(
)
Received:
2024-04-17
Revised:
2024-06-21
Online:
2024-08-25
Published:
2024-08-21
Contact:
QIN Lei, WANG Yong
ZHAO Jingyi, WU Xiaoxu, HU Yunjie, GAI Shuting, ZHU Zhihao, QIN Lei, WANG Yong. Molecular Cloning and Functional Analysis of AcGAI in Onion Flowering Regulation[J]. Acta Horticulturae Sinica, 2024, 51(8): 1792-1802.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0385
用途 Use | 引物名称 Primer name | 引物序列(5′-3′) Primer sequence |
---|---|---|
基因克隆 Gene of cloning | GAI-S | CGGGATCCATGATCGATCCATTTCTGTTC |
GAI-X | CGAGCTCCTACAGCAGGTTACTGACATTG | |
酵母双杂交 | GAI-JS | CCGGAATTCATGATCGATCCATTTCTGTTC |
Yeast two-hybrid assay | GAI-JX | CGGGATCCCTACAGCAGGTTACTGACATTG |
CO-JS-AD | GCCATGGAGGCCAGTGAATTCATGTATGCTGAGCCTAGGCC | |
CO-JX-AD | AGCTCGAGCTCGATGGATCCTTACTTATTTTGTGATTTTGTGAC | |
双分子荧光互补 | C-GAI-F | TGGCGCGCCACTAGTGGATCCATGATCGATCCATTTCTGT |
Bimolecular fluorescent complimentary | C-GAI-R | CCCGGGAGCGGTACCCTCGAGCAGCAGGTTACTGACATT |
N-CO-F | TGGCGCGCCACTAGTGGATCCATGTATGCTGAGCCTAGG | |
N-CO-R | CCCGGGAGCGGTACCCTCGAGCTTATTTTGTGATTTTGTGACTT | |
qRT-PCR | AtGAI-F | GGACCTGACCGAGTTGAGCG |
AtGAI-R | AGTTTCCAAGCCGAGGTGGC | |
AtCO-F | ACTACTCACCACCAAAGCGA | |
AtCO-R | GAAGCAACCTCCTTGGCATC | |
AtFT-F | TGGAGACGTTCTTGATCCGT | |
AtFT-R | CCTGAGGTCTTCTCCACCAA | |
AcActin-F | ACACGGCCTGGATAGCAACAT | |
AcActin-R | AGAGCAGTATTCCCAAGCATT |
Table 1 Primer sequence used in this study
用途 Use | 引物名称 Primer name | 引物序列(5′-3′) Primer sequence |
---|---|---|
基因克隆 Gene of cloning | GAI-S | CGGGATCCATGATCGATCCATTTCTGTTC |
GAI-X | CGAGCTCCTACAGCAGGTTACTGACATTG | |
酵母双杂交 | GAI-JS | CCGGAATTCATGATCGATCCATTTCTGTTC |
Yeast two-hybrid assay | GAI-JX | CGGGATCCCTACAGCAGGTTACTGACATTG |
CO-JS-AD | GCCATGGAGGCCAGTGAATTCATGTATGCTGAGCCTAGGCC | |
CO-JX-AD | AGCTCGAGCTCGATGGATCCTTACTTATTTTGTGATTTTGTGAC | |
双分子荧光互补 | C-GAI-F | TGGCGCGCCACTAGTGGATCCATGATCGATCCATTTCTGT |
Bimolecular fluorescent complimentary | C-GAI-R | CCCGGGAGCGGTACCCTCGAGCAGCAGGTTACTGACATT |
N-CO-F | TGGCGCGCCACTAGTGGATCCATGTATGCTGAGCCTAGG | |
N-CO-R | CCCGGGAGCGGTACCCTCGAGCTTATTTTGTGATTTTGTGACTT | |
qRT-PCR | AtGAI-F | GGACCTGACCGAGTTGAGCG |
AtGAI-R | AGTTTCCAAGCCGAGGTGGC | |
AtCO-F | ACTACTCACCACCAAAGCGA | |
AtCO-R | GAAGCAACCTCCTTGGCATC | |
AtFT-F | TGGAGACGTTCTTGATCCGT | |
AtFT-R | CCTGAGGTCTTCTCCACCAA | |
AcActin-F | ACACGGCCTGGATAGCAACAT | |
AcActin-R | AGAGCAGTATTCCCAAGCATT |
Fig. 1 Amino acid sequence alignment of AcGAI from Allium cepa and GAI proteins of other plants Vv:Vitis vinifera;Sl:Solanum lycopersicum;St:Solanum tuberosum;Ps:Pisum sativum;Ca:Cicer arietinum;Mt:Medicago truncatula;Gm:Glycine max;Va:Vigna angularis;Pv:Phaseolus vulgaris;Si:Sesamum indicum;Gh:Gossypium hirsutum;Pp:Pyrus pyrifolia;Md:Malus × domestica;Rc:Ricinus communis;Cs:Cucumis sativus;Aa:Ambrosia artemisiifolia;At:Arabidopsis thaliana;Ac:Allium cepa.
Fig. 2 Phylogenetic analysis of AcGAI from Allium cepa and GAI proteins of other plants Vv:Vitis vinifera;Aa:Ambrosia artemisiifolia;At:Arabidopsis thaliana;Ac:Allium cepa;Si:Sesamum indicum;Sl:Solanum lycopersicum;St:Solanum tuberosum;Ps:Pisum sativum;Ca:Cicer arietinum;Mt:Medicago truncatula;Gm:Glycine max;Va:Vigna angularis;Pv:Phaseolus vulgaris;Cs:Cucumis sativus;Gh:Gossypium hirsutum;Rc:Ricinus communis;Pp:Pyrus pyrifolia;Md:Malus × domestica.
Fig. 3 Expression analysis of AcGAI at different tissues in Allium cepa Different lowercase letters indicate one-way analysis of variance(ANOVA),P < 0.05.
Fig. 4 Changes in phenotype and expression of related endogenous genes in Arabidopsis thaliana wild type(WT)and overexpressing AcGAI(WT-AcGAI-OE) Signifcant difference between treatment and control at same time point were analyszed by using t-test. **P < 0.01. The same below.
[1] |
doi: 10.1111/j.1365-313X.2010.04148.x pmid: 20409274 |
[2] |
|
[3] |
|
白云赫, 朱旭东, 樊秀彩, 王晨, 张文颖, 刘崇怀, 房经贵. 2019. 植物DELLA蛋白及其应答赤霉素信号调控植物生长发育的研究进展. 分子植物育种, 17 (8):2509-2516.
|
|
[4] |
|
[5] |
|
[6] |
|
[7] |
doi: 10.1093/plcell/koab102 pmid: 33822231 |
[8] |
|
刘杭, 杨贺忠, 李亮, 王芳, 吴少华, 李永裕. 2016. 梨休眠DELLA蛋白PpGAI基因的克隆与表达分析. 分子植物育种, 14 (8):1995-2002.
|
|
[9] |
|
罗靖. 2013. 生长抑制子RhGAI1在乙烯调节的月季花瓣扩展过程中的功能分析[博士论文]. 北京: 中国农业大学.
|
|
[10] |
|
[11] |
|
[12] |
|
曲廷云, 王拴锁, 彭明, 崔百明, 郑银英. 2010. 海岛棉DELLA蛋白编码基因GbGAI的克隆与功能初步分析. 中国农学通报, 26 (13):30-35.
|
|
[13] |
|
盛洁, 杨翠翠, 吴小旭, 袁巧玲, 陈典, 张东源, 王勇. 2018. 洋葱光周期途径转录因子基因AcCOL7的克隆及功能鉴定. 园艺学报, 45 (3):493-502.
doi: 10.16420/j.issn.0513-353x.2017-0810 |
|
[14] |
|
宋仙萍. 2013. 大豆DELLA基因GmGAI1α参与赤霉素信号传导途径的功能分析[硕士论文]. 哈尔滨: 东北农业大学.
|
|
[15] |
doi: 10.1007/s00018-011-0673-y pmid: 21611891 |
[16] |
|
[17] |
|
[18] |
|
[19] |
|
[20] |
|
[21] |
|
[22] |
|
[23] |
|
张颜. 2014. 黄瓜中GA信号转导因子CsGAIP和CsGAMYB1的克隆与功能分析[博士论文]. 北京: 中国农业大学.
|
|
[24] |
|
张涵, 王学敏, 刘希强, 马琳, 温红雨, 王赞. 2019. 紫花苜蓿MsGAI的克隆、表达及遗传转化. 中国农业科学, 52 (2):201-214.
doi: 10.3864/j.issn.0578-1752.2019.02.002 |
|
[25] |
|
赵春丽, 王晓, 陈家兰, 陈何, 王乐, 赖钟雄, 刘生财. 2020. 植物DELLA蛋白家族研究进展. 应用与环境生物学报, 26 (5):1299-1308.
|
|
[26] |
|
周鹏, 李钱峰, 熊敏, 范晓磊, 赵冬生, 张昌泉, 刘巧泉. 2020. DELLA蛋白介导的激素互作调控植物生长发育研究进展. 植物生理学报, 56 (4):661-671.
|
[1] | ZHANG Yue, ZHANG Yunchun, DANG Jiangbo, LIN Shoukai, WU Di, JING Danlong, GUO Qigao, LIANG Guolu, XIANG Suqiong. A Review of Inflorescence Development and Formation Mechanism of Loquat [J]. Acta Horticulturae Sinica, 2023, 50(9): 1929-1943. |
[2] | CHEN Xinchen, ZHAO Huimin, WANG Sen, WU Simin, XIANG Lin, CHAN Zhulong, WANG Yanping. Analysis of Floral Bud Differentiation and Related Genes Expression Under Different Temperatures in Tulipa gesneriana [J]. Acta Horticulturae Sinica, 2023, 50(5): 1037-1047. |
[3] | XUE Yuqian, LIU Zhiyong, SUN Kairong, ZHANG Xiuxin, LÜ Yingmin, XUE Jingqi. The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture [J]. Acta Horticulturae Sinica, 2023, 50(3): 596-606. |
[4] | NIU Xiqaing, LUO Xiaoyun, KANG Kaicheng, HUANG Xianzhong, HU Nengbing, SUI Yihu, AI Hao. Genome-wide Identification,Comparative Evolution and Expression Analysis of PEBP Gene Family from Capsicum annuum [J]. Acta Horticulturae Sinica, 2021, 48(5): 947-959. |
[5] | LI Chonghui, YANG Guangsui, ZHANG Zhiqun, YIN Junmei. A Novel R2R3-MYB Transcription Factor Gene AaMYB6 Involved in Anthocyanin Biosynthesis in Anthurium andraeanum [J]. Acta Horticulturae Sinica, 2021, 48(10): 1859-1872. |
[6] | WANG Xiaoquan*,LI Shengnan*,ZHANG Junli*,WANG Zhimin,WEI Dayong,and TANG Qinglin**. Progress in Regulation Mechanism of Inflorescence Development in Arabidopsis and Cruciferous Vegetables [J]. ACTA HORTICULTURAE SINICA, 2018, 45(9): 1727-1738. |
[7] | HE Chongdan1,2,CAI Yumeng2,LI Meng1,2,DONG Jinjin2,LIU Wei2,WANG Junyi2,and WANG Yiqiang1,2,*. Screening and Expression Analysis of Related Genes Based on Transcriptome Sequencing of Ginkgo Flower Buds at Three Differentiation Stages [J]. ACTA HORTICULTURAE SINICA, 2018, 45(8): 1479-1490. |
[8] | LI Changman1,2,*,LI Zhaochuang2,*,WANG Zhimin2,SONG Ming2,and TANG Qinglin2,**. Progress in Mechanisms of Floral Inhibiting Factor AGL18 in Regulating Flowering Time of Arabidopsis and Mustard [J]. ACTA HORTICULTURAE SINICA, 2017, 44(9): 1717-1729. |
[9] | HE Junhu1,LI Weizheng1,2,*,CHEN Huarui1,ZHAO Xiaoqing1,and ZHU Min1. Cloning of the Light Harvesting Chlorophyll a/b Binding Protein Gene (MiCAB2)from‘Jinhuang’Mango,and Correlation Analysis Between Its Expression and Flowering Regulation [J]. ACTA HORTICULTURAE SINICA, 2017, 44(7): 1275-1286. |
[10] | LI Chaochuang1,MA Guanpeng1,2,XIE Ting1,3,CHEN Jiao1,WANG Zhimin1,SONG Ming1,and TANG Qinglin1,*. Analysis of Interactions Between AGL18 Family Members and Flowering Time Integrator Factor SOC1in Brassica juncea [J]. ACTA HORTICULTURAE SINICA, 2017, 44(3): 463-474. |
[11] | DUAN Xuwei,NI Yang,ZHANG Kaichun*,ZHANG Xiaoming,YAN Guohua,WANG Jing,and. The Flowering-related Genes Expression Differences in Different Scionstock#br# Combination of Cherry [J]. ACTA HORTICULTURAE SINICA, 2017, 44(11): 2064-2074. |
[12] | JIANG Wei*,ZHOU Wenwen*,LI Zhaochuang,YAN Kai,WANG Yu,WANG Zhimin,SONG Ming,and TANG Qinglin**. Interactions of Flowering Promoting Factor AGL19 with Integrator Factors AGL24 and SOC1 in Brassica oleracea var. italica [J]. ACTA HORTICULTURAE SINICA, 2017, 44(10): 1905-1913. |
[13] | LI Chao-chuang,MA Guan-peng,YANG Xiu-qin,WANG Zhi-min,SONG Ming,and TANG Qing-lin. Expression Analysis of Flowering Regulation Factor SVP and Identification of Mediation Sites of SVP/FLC in Brassica juncea [J]. ACTA HORTICULTURAE SINICA, 2016, 43(8): 1513-1524. |
[14] | SONG Cai, WU Yun-Ta-Na, LI Hui, XU Jing-Shi, LIU Ru-Qing. The Analysis of Digital Gene Expression About Kernel Apricot Flower Buds at Three Development Stages [J]. ACTA HORTICULTURAE SINICA, 2015, 42(8): 1559-1568. |
[15] | LIU Zhi-Yu, YANG Pu-Li, JIANG Wei, GU Hui-Ying, WANG Zhi-Min, SONG Ming, TANG Qing-Lin. Differences of FLC Family Members and Its Effect on Dimerization of FLC/SVP in Brassica oleracea [J]. ACTA HORTICULTURAE SINICA, 2014, 41(5): 869-880. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Copyright © 2012 Acta Horticulturae Sinica 京ICP备10030308号-2 国际联网备案号 11010802023439
Tel: 010-82109523 E-Mail: yuanyixuebao@126.com
Support by: Beijing Magtech Co.Ltd