Screening Proteins of Nicotiana occidentalis That Interact with Coat Protein of Apple Stem Pitting Virus Using Yeast Two-hybrid System

doi:10.16420/j.issn.0513-353x.2022-0507

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (11): 2509-2515.doi: 10.16420/j.issn.0513-353x.2022-0507

• Plant Protection • Previous Articles Next Articles

Screening Proteins of Nicotiana occidentalis That Interact with Coat Protein of Apple Stem Pitting Virus Using Yeast Two-hybrid System

DING Lei¹^,², SUN Pingping¹, ZHANG Lei¹^,^*(), LI Zhengnan¹^,^*()

¹ College of Horticulture and Plant Protection，Inner Mongolia Agricultural University，Hohhot 010018，China
² Mianyang Academy of Agricultural Sciences，Mianyang，Sichuan 621023，China

Received:2023-02-01 Revised:2023-09-02 Online:2023-11-25 Published:2023-11-28
Contact: ZHANG Lei, LI Zhengnan

Abstract

Abstract:

Apple stem pitting virus（ASPV）is a latent virus on apple and pear trees worldwide. Nicotiana occidentalis is an important herbaceous host plant for it. In this study，ASPV coat protein（CP）was used as bait to screen a cDNA library of N. occidentalis. Thirty proteins that interact with ASPV CP were obtained by yeast two-hybrid co-transformation and NCBI BLAST. This study could be useful for understanding the molecular mechanism of ASPV infection in N. occidentalis and the function of CP in the process of virus infection.

Key words: yeast two-hybrid, coat protein, apple stem pitting virus, protein interaction

DING Lei, SUN Pingping, ZHANG Lei, LI Zhengnan. Screening Proteins of Nicotiana occidentalis That Interact with Coat Protein of Apple Stem Pitting Virus Using Yeast Two-hybrid System[J]. Acta Horticulturae Sinica, 2023, 50(11): 2509-2515.

Figures/Tables 5

References 25

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序号 No.	候选蛋白 Putative protein	物种 Species	GenBank登录号 GenBank accession No.	总分值 Total score	覆盖率/% Query cover	E值 E-value	序列一致性/% Sequence identity
1	40S ribosomal protein	野生烟草Nicotiana attenuata	XM_019380942.1	314	74	0	95.40
2	Putative dual specificity protein	绒毛状烟草N. tomentosiformis	XM_009598741.3	1 831	94	0	95.79
3	Putative late blight resistance protein	美花烟草N. sylvestris	XM_009777746.1	1 419	82	0	94.17
4	Fructose-1,6-bisphosphatase	普通烟草N. tabacum	XM_016645668.1	1 962	93	0	97.16
5	Transmembrane protein	普通烟草N. tabacum	XM_016608120.1	1 541	94	0	93.88
6	DEAD-box ATP-dependent RNA helicase	普通烟草N. tabacum	XM_016614675.1	1 652	86	0	95.38
7	Acetolactate synthase	野生烟草N. attenuata	XM_019368947.1	1 552	92	0	97.99
8	Chlorophyll a-b binding protein	美花烟草N. sylvestris	XM_009797464.1	1 611	84	0	97.66
9	Lysine--tRNA ligase	野生烟草N. attenuata	XM_019388504.1	1 877	95	0	96.12
10	L-ascorbate peroxidase 2	绒毛状烟草N. tomentosiformis	XM_009608760.3	1 644	89	0	95.63
11	Photosystem I reaction center subunit III	普通烟草N. tabacum	XM_016584537.1	1 321	86	0	97.07
12	Ribulose bisphosphate carboxylase small chain	野生烟草N. attenuata	XM_019393743.1	1 206	86	0	94.63
13	60S ribosomal protein	普通烟草N. tabacum	XM_016621890.1	1 184	79	0	91.28
14	Probable ubiquitin-like-specific protease	野生烟草N. attenuata	XM_019370696.1	507	55	7e-139	95.33
15	Phototropin	普通烟草N. tabacum	XM_016615622.1	1 075	61	0	93.62
16	Ras-related protein	绒毛状烟草N. tomentosiformis	XM_009613476.3	1 544	90	0	94.64
17	Persulfide dioxygenase	野生烟草N. attenuata	XM_019394068.1	424	76	1e-113	78.43
18	Fructose-bisphosphate aldolase	野生烟草N. attenuata	XM_019380711.1	1 537	93	0	98.07
19	Peroxidase	野生烟草N. attenuata	XM_019378242.1	1 038	87	0	90.70
20	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	野生烟草N. attenuata	XM_019383154.1	1 557	93	0	98.53
21	Riboflavin synthase	普通烟草N. tabacum	XM_016652029.1	676	86	0	93.42
22	Cytosolic Fe-S cluster assembly factor	普通烟草N. tabacum	XM_016639050.1	1 526	94	0	95.68
23	Probable ribose-5-phosphate isomerase	普通烟草N. tabacum	XM_016602671.1	1 517	90	0	94.96
24	Proline synthase co-transcribed bacterial homolog protein	野生烟草N. attenuata	XM_019396117.1	715	86	0	95.14
25	L-ascorbate peroxidase 1	普通烟草N. tabacum	XR_001646090.1	780	59	0	96.41
26	Phosphoglycerate kinase	绒毛状烟草N. tomentosiformis	XM_009623760.3	817	71	0	96.73
27	DNA-directed RNA polymerase	野生烟草N. attenuata	XM_019380496.1	1 565	93	0	97.00
28	WD repeat-containing protein	美花烟草N. sylvestris	XM_009763517.1	1 291	79	0	97.13
29	Chloroplast PsbP2 precursor	本氏烟草N. benthamiana	JF897608.1	1 469	93	0	97.15
30	mRNA for aquaporin	普通烟草N. tabacum	AJ001416.1	1 718	92	0	94.29

序号 No.	候选蛋白 Putative protein	物种 Species	GenBank登录号 GenBank accession No.	总分值 Total score	覆盖率/% Query cover	E值 E-value	序列一致性/% Sequence identity
1	40S ribosomal protein	野生烟草Nicotiana attenuata	XM_019380942.1	314	74	0	95.40
2	Putative dual specificity protein	绒毛状烟草N. tomentosiformis	XM_009598741.3	1 831	94	0	95.79
3	Putative late blight resistance protein	美花烟草N. sylvestris	XM_009777746.1	1 419	82	0	94.17
4	Fructose-1,6-bisphosphatase	普通烟草N. tabacum	XM_016645668.1	1 962	93	0	97.16
5	Transmembrane protein	普通烟草N. tabacum	XM_016608120.1	1 541	94	0	93.88
6	DEAD-box ATP-dependent RNA helicase	普通烟草N. tabacum	XM_016614675.1	1 652	86	0	95.38
7	Acetolactate synthase	野生烟草N. attenuata	XM_019368947.1	1 552	92	0	97.99
8	Chlorophyll a-b binding protein	美花烟草N. sylvestris	XM_009797464.1	1 611	84	0	97.66
9	Lysine--tRNA ligase	野生烟草N. attenuata	XM_019388504.1	1 877	95	0	96.12
10	L-ascorbate peroxidase 2	绒毛状烟草N. tomentosiformis	XM_009608760.3	1 644	89	0	95.63
11	Photosystem I reaction center subunit III	普通烟草N. tabacum	XM_016584537.1	1 321	86	0	97.07
12	Ribulose bisphosphate carboxylase small chain	野生烟草N. attenuata	XM_019393743.1	1 206	86	0	94.63
13	60S ribosomal protein	普通烟草N. tabacum	XM_016621890.1	1 184	79	0	91.28
14	Probable ubiquitin-like-specific protease	野生烟草N. attenuata	XM_019370696.1	507	55	7e-139	95.33
15	Phototropin	普通烟草N. tabacum	XM_016615622.1	1 075	61	0	93.62
16	Ras-related protein	绒毛状烟草N. tomentosiformis	XM_009613476.3	1 544	90	0	94.64
17	Persulfide dioxygenase	野生烟草N. attenuata	XM_019394068.1	424	76	1e-113	78.43
18	Fructose-bisphosphate aldolase	野生烟草N. attenuata	XM_019380711.1	1 537	93	0	98.07
19	Peroxidase	野生烟草N. attenuata	XM_019378242.1	1 038	87	0	90.70
20	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	野生烟草N. attenuata	XM_019383154.1	1 557	93	0	98.53
21	Riboflavin synthase	普通烟草N. tabacum	XM_016652029.1	676	86	0	93.42
22	Cytosolic Fe-S cluster assembly factor	普通烟草N. tabacum	XM_016639050.1	1 526	94	0	95.68
23	Probable ribose-5-phosphate isomerase	普通烟草N. tabacum	XM_016602671.1	1 517	90	0	94.96
24	Proline synthase co-transcribed bacterial homolog protein	野生烟草N. attenuata	XM_019396117.1	715	86	0	95.14
25	L-ascorbate peroxidase 1	普通烟草N. tabacum	XR_001646090.1	780	59	0	96.41
26	Phosphoglycerate kinase	绒毛状烟草N. tomentosiformis	XM_009623760.3	817	71	0	96.73
27	DNA-directed RNA polymerase	野生烟草N. attenuata	XM_019380496.1	1 565	93	0	97.00
28	WD repeat-containing protein	美花烟草N. sylvestris	XM_009763517.1	1 291	79	0	97.13
29	Chloroplast PsbP2 precursor	本氏烟草N. benthamiana	JF897608.1	1 469	93	0	97.15
30	mRNA for aquaporin	普通烟草N. tabacum	AJ001416.1	1 718	92	0	94.29

Screening Proteins of Nicotiana occidentalis That Interact with Coat Protein of Apple Stem Pitting Virus Using Yeast Two-hybrid System

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