Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (11): 2509-2515.doi: 10.16420/j.issn.0513-353x.2022-0507
• Plant Protection • Previous Articles Next Articles
DING Lei1,2, SUN Pingping1, ZHANG Lei1,*(), LI Zhengnan1,*(
)
Received:
2023-02-01
Revised:
2023-09-02
Online:
2023-11-25
Published:
2023-11-28
Contact:
ZHANG Lei, LI Zhengnan
DING Lei, SUN Pingping, ZHANG Lei, LI Zhengnan. Screening Proteins of Nicotiana occidentalis That Interact with Coat Protein of Apple Stem Pitting Virus Using Yeast Two-hybrid System[J]. Acta Horticulturae Sinica, 2023, 50(11): 2509-2515.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0507
序号 No. | 候选蛋白 Putative protein | 物种 Species | GenBank登录号 GenBank accession No. | 总分值 Total score | 覆盖 率/% Query cover | E值 E-value | 序列一 致性/% Sequence identity |
---|---|---|---|---|---|---|---|
1 | 40S ribosomal protein | 野生烟草Nicotiana attenuata | XM_019380942.1 | 314 | 74 | 0 | 95.40 |
2 | Putative dual specificity protein | 绒毛状烟草N. tomentosiformis | XM_009598741.3 | 1 831 | 94 | 0 | 95.79 |
3 | Putative late blight resistance protein | 美花烟草N. sylvestris | XM_009777746.1 | 1 419 | 82 | 0 | 94.17 |
4 | Fructose-1,6-bisphosphatase | 普通烟草N. tabacum | XM_016645668.1 | 1 962 | 93 | 0 | 97.16 |
5 | Transmembrane protein | 普通烟草N. tabacum | XM_016608120.1 | 1 541 | 94 | 0 | 93.88 |
6 | DEAD-box ATP-dependent RNA helicase | 普通烟草N. tabacum | XM_016614675.1 | 1 652 | 86 | 0 | 95.38 |
7 | Acetolactate synthase | 野生烟草N. attenuata | XM_019368947.1 | 1 552 | 92 | 0 | 97.99 |
8 | Chlorophyll a-b binding protein | 美花烟草N. sylvestris | XM_009797464.1 | 1 611 | 84 | 0 | 97.66 |
9 | Lysine--tRNA ligase | 野生烟草N. attenuata | XM_019388504.1 | 1 877 | 95 | 0 | 96.12 |
10 | L-ascorbate peroxidase 2 | 绒毛状烟草N. tomentosiformis | XM_009608760.3 | 1 644 | 89 | 0 | 95.63 |
11 | Photosystem I reaction center subunit III | 普通烟草N. tabacum | XM_016584537.1 | 1 321 | 86 | 0 | 97.07 |
12 | Ribulose bisphosphate carboxylase small chain | 野生烟草N. attenuata | XM_019393743.1 | 1 206 | 86 | 0 | 94.63 |
13 | 60S ribosomal protein | 普通烟草N. tabacum | XM_016621890.1 | 1 184 | 79 | 0 | 91.28 |
14 | Probable ubiquitin-like-specific protease | 野生烟草N. attenuata | XM_019370696.1 | 507 | 55 | 7e-139 | 95.33 |
15 | Phototropin | 普通烟草N. tabacum | XM_016615622.1 | 1 075 | 61 | 0 | 93.62 |
16 | Ras-related protein | 绒毛状烟草N. tomentosiformis | XM_009613476.3 | 1 544 | 90 | 0 | 94.64 |
17 | Persulfide dioxygenase | 野生烟草N. attenuata | XM_019394068.1 | 424 | 76 | 1e-113 | 78.43 |
18 | Fructose-bisphosphate aldolase | 野生烟草N. attenuata | XM_019380711.1 | 1 537 | 93 | 0 | 98.07 |
19 | Peroxidase | 野生烟草N. attenuata | XM_019378242.1 | 1 038 | 87 | 0 | 90.70 |
20 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 野生烟草N. attenuata | XM_019383154.1 | 1 557 | 93 | 0 | 98.53 |
21 | Riboflavin synthase | 普通烟草N. tabacum | XM_016652029.1 | 676 | 86 | 0 | 93.42 |
22 | Cytosolic Fe-S cluster assembly factor | 普通烟草N. tabacum | XM_016639050.1 | 1 526 | 94 | 0 | 95.68 |
23 | Probable ribose-5-phosphate isomerase | 普通烟草N. tabacum | XM_016602671.1 | 1 517 | 90 | 0 | 94.96 |
24 | Proline synthase co-transcribed bacterial homolog protein | 野生烟草N. attenuata | XM_019396117.1 | 715 | 86 | 0 | 95.14 |
25 | L-ascorbate peroxidase 1 | 普通烟草N. tabacum | XR_001646090.1 | 780 | 59 | 0 | 96.41 |
26 | Phosphoglycerate kinase | 绒毛状烟草N. tomentosiformis | XM_009623760.3 | 817 | 71 | 0 | 96.73 |
27 | DNA-directed RNA polymerase | 野生烟草N. attenuata | XM_019380496.1 | 1 565 | 93 | 0 | 97.00 |
28 | WD repeat-containing protein | 美花烟草N. sylvestris | XM_009763517.1 | 1 291 | 79 | 0 | 97.13 |
29 | Chloroplast PsbP2 precursor | 本氏烟草N. benthamiana | JF897608.1 | 1 469 | 93 | 0 | 97.15 |
30 | mRNA for aquaporin | 普通烟草N. tabacum | AJ001416.1 | 1 718 | 92 | 0 | 94.29 |
Table 1 Putative proteins screened in the cDNA library of Nicotiana occidentalis interacting with ASPV CP
序号 No. | 候选蛋白 Putative protein | 物种 Species | GenBank登录号 GenBank accession No. | 总分值 Total score | 覆盖 率/% Query cover | E值 E-value | 序列一 致性/% Sequence identity |
---|---|---|---|---|---|---|---|
1 | 40S ribosomal protein | 野生烟草Nicotiana attenuata | XM_019380942.1 | 314 | 74 | 0 | 95.40 |
2 | Putative dual specificity protein | 绒毛状烟草N. tomentosiformis | XM_009598741.3 | 1 831 | 94 | 0 | 95.79 |
3 | Putative late blight resistance protein | 美花烟草N. sylvestris | XM_009777746.1 | 1 419 | 82 | 0 | 94.17 |
4 | Fructose-1,6-bisphosphatase | 普通烟草N. tabacum | XM_016645668.1 | 1 962 | 93 | 0 | 97.16 |
5 | Transmembrane protein | 普通烟草N. tabacum | XM_016608120.1 | 1 541 | 94 | 0 | 93.88 |
6 | DEAD-box ATP-dependent RNA helicase | 普通烟草N. tabacum | XM_016614675.1 | 1 652 | 86 | 0 | 95.38 |
7 | Acetolactate synthase | 野生烟草N. attenuata | XM_019368947.1 | 1 552 | 92 | 0 | 97.99 |
8 | Chlorophyll a-b binding protein | 美花烟草N. sylvestris | XM_009797464.1 | 1 611 | 84 | 0 | 97.66 |
9 | Lysine--tRNA ligase | 野生烟草N. attenuata | XM_019388504.1 | 1 877 | 95 | 0 | 96.12 |
10 | L-ascorbate peroxidase 2 | 绒毛状烟草N. tomentosiformis | XM_009608760.3 | 1 644 | 89 | 0 | 95.63 |
11 | Photosystem I reaction center subunit III | 普通烟草N. tabacum | XM_016584537.1 | 1 321 | 86 | 0 | 97.07 |
12 | Ribulose bisphosphate carboxylase small chain | 野生烟草N. attenuata | XM_019393743.1 | 1 206 | 86 | 0 | 94.63 |
13 | 60S ribosomal protein | 普通烟草N. tabacum | XM_016621890.1 | 1 184 | 79 | 0 | 91.28 |
14 | Probable ubiquitin-like-specific protease | 野生烟草N. attenuata | XM_019370696.1 | 507 | 55 | 7e-139 | 95.33 |
15 | Phototropin | 普通烟草N. tabacum | XM_016615622.1 | 1 075 | 61 | 0 | 93.62 |
16 | Ras-related protein | 绒毛状烟草N. tomentosiformis | XM_009613476.3 | 1 544 | 90 | 0 | 94.64 |
17 | Persulfide dioxygenase | 野生烟草N. attenuata | XM_019394068.1 | 424 | 76 | 1e-113 | 78.43 |
18 | Fructose-bisphosphate aldolase | 野生烟草N. attenuata | XM_019380711.1 | 1 537 | 93 | 0 | 98.07 |
19 | Peroxidase | 野生烟草N. attenuata | XM_019378242.1 | 1 038 | 87 | 0 | 90.70 |
20 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 野生烟草N. attenuata | XM_019383154.1 | 1 557 | 93 | 0 | 98.53 |
21 | Riboflavin synthase | 普通烟草N. tabacum | XM_016652029.1 | 676 | 86 | 0 | 93.42 |
22 | Cytosolic Fe-S cluster assembly factor | 普通烟草N. tabacum | XM_016639050.1 | 1 526 | 94 | 0 | 95.68 |
23 | Probable ribose-5-phosphate isomerase | 普通烟草N. tabacum | XM_016602671.1 | 1 517 | 90 | 0 | 94.96 |
24 | Proline synthase co-transcribed bacterial homolog protein | 野生烟草N. attenuata | XM_019396117.1 | 715 | 86 | 0 | 95.14 |
25 | L-ascorbate peroxidase 1 | 普通烟草N. tabacum | XR_001646090.1 | 780 | 59 | 0 | 96.41 |
26 | Phosphoglycerate kinase | 绒毛状烟草N. tomentosiformis | XM_009623760.3 | 817 | 71 | 0 | 96.73 |
27 | DNA-directed RNA polymerase | 野生烟草N. attenuata | XM_019380496.1 | 1 565 | 93 | 0 | 97.00 |
28 | WD repeat-containing protein | 美花烟草N. sylvestris | XM_009763517.1 | 1 291 | 79 | 0 | 97.13 |
29 | Chloroplast PsbP2 precursor | 本氏烟草N. benthamiana | JF897608.1 | 1 469 | 93 | 0 | 97.15 |
30 | mRNA for aquaporin | 普通烟草N. tabacum | AJ001416.1 | 1 718 | 92 | 0 | 94.29 |
Fig. 4 Confirmation of positive interactors P:Positive control;N:Negative control;1-30:The proteins screened from the cDNA library of N. occidentalis.
[1] |
doi: 10.1007/s00705-004-0304-0 pmid: 15098118 |
[2] |
|
[3] |
|
[4] |
doi: 10.1111/j.1365-3059.2009.02164.x |
[5] |
doi: 10.1016/j.bbadis.2008.08.010 URL |
[6] |
|
[7] |
doi: 10.1038/340245a0 |
[8] |
|
高德航, 何成勇, 邢飞, 侯婉莹, 李世访, 战斌慧, 王红清. 2021. 苹果茎痘病毒在山楂中的首次鉴定与序列分析. 植物病理学报, 51 (4):515-524.
|
|
[9] |
doi: 10.1016/j.coviro.2012.10.001 pmid: 23122854 |
[10] |
doi: 10.1099/0022-1317-75-7-1535 URL |
[11] |
doi: 10.1016/j.virusres.2008.07.028 URL |
[12] |
doi: 10.1094/PHYTO.2001.91.11.1085 pmid: 18943445 |
[13] |
doi: 10.1046/j.1365-313X.1994.6050637.x URL |
[14] |
doi: 10.1007/s12298-019-00693-1 |
[15] |
|
李丽丽, 董雅凤, 张尊平, 张志宏, 范旭东, 裴光前. 2010. 苹果茎痘病毒RT-PCR检测及分子变异分析. 园艺学报, 37 (1):9-16.
|
|
[16] |
|
[17] |
pmid: 9687881 |
[18] |
|
[19] |
|
[20] |
doi: 10.16420/j.issn.0513-353x.2018-1074 URL |
孙平平, 鞠明岫, 马强, 李正男. 2019. 苹果茎痘病毒内蒙古分离物基因组序列和生物学特征研究. 园艺学报, 46 (10):2037-2046.
doi: 10.16420/j.issn.0513-353x.2018-1074 URL |
|
[21] |
|
[22] |
doi: 10.1016/j.virusres.2014.11.014 pmid: 25445337 |
[23] |
|
[24] |
|
叶朝燕, 张阳, 汪冒君, 吴军, 陈卫良. 2015. 镰刀菌乙酰乳酸合成酶抑制剂的筛选及抗菌性分析. 浙江大学学报(理学版), 42 (2):166-171.
|
|
[25] |
|
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