Identification of Cucumber mosaic virus Infecting Pandanus tectorius

doi:10.16420/j.issn.0513-353x.2018-1003

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (10): 2055-2060.doi: 10.16420/j.issn.0513-353x.2018-1003

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Identification of Cucumber mosaic virus Infecting Pandanus tectorius

WANG Kaili1,2，ZHANG Jie2，WU Kuo2，YANG Xuebao3，WEI Jianli2，WANG Changming1,*，and DONG Jiahong2,4,*

1Southwest Forestry University，College of Life Sciences，Kunming 650224，China；2Biotechnology and Germplasm Resources Institute，Yunnan Academy of Agricultural Sciences，Kunming 650223，China；3Yuanjiang County Agricultural Technology Extension Station，Yuxi 653100，China；4College of Traditional Chinese Pharmacy，Yunnan University of Chinese Medicine，Kunming 650500，China

Online:2019-10-25 Published:2019-10-25

Abstract

Abstract: In order to confirm the viral pathogen causing yellowing and mosaic spots symptoms in the leaves of Pandanus tectorius in Yuanjiang County，Yunnan Province. Electron microscopy revealed that the virus in the dips of the diseased leaves has icosahedral particles ca. 30 nm in diameter. With the specific primer pairs for coat protein（CP）gene sequence of Cucumber mosaic virus（CMV），RT-PCR was used to detect the viral pathogen infection to P. tectorius and the target fragment was cloned and sequenced. The sequence analysis showed that it had the highest identity of 98% with CP gene sequence of CMV isolates from Indian，Sichuan and Thailand. This isolate belongs to CMV subgroup I. It is the first report of infection of P. tectorius by a viral pathogen.

Key words: Pandanus tectorius, Cucumber mosaic virus, viral particle, coat protein gene, infection

CLC Number:

S 68

WANG Kaili1,2，ZHANG Jie2，WU Kuo2，YANG Xuebao3，WEI Jianli2，WANG Changming1,*，and DONG Jiahong2,4,*. Identification of Cucumber mosaic virus Infecting Pandanus tectorius[J]. ACTA HORTICULTURAE SINICA, 2019, 46(10): 2055-2060.

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Identification of Cucumber mosaic virus Infecting Pandanus tectorius

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