Abstract: The purpose of this study was to investigate effects of sterilized and non sterilized matur vinasse and Distiller’s grains on the Malus hupehensis Rehd. and replanted soil microorganisms. The study showed that lees（vinasse and distiller’s grains）increased biomass of seedlings of Malus hupehensis Rehd. It could enhance Malus hupeheusis Rehd. seedlings biomass. Plant height，fresh weight and dry weight after adding vinasse and distiller’s grains. And the number of bacteria and fungi in the soil with continuous cropping on the photographic is also improved significantly after using them. Based on the cluster analysis. PCA and diversity index analysis，the soil microbial community structure had obvious changes.In different types and different processing mode of lees microbial community structure was different. The dominant population presented in the soil and changed microbial community diversity index and richness index by reason of lees carred and sterile lees breeding in the soil. Through the real time fluorescence quantitative of Fusarium proliferatum in soil，the results show that Fusarium proliferatum copy number of vinasse，distiller’s grains，sterile vinasse and distiller’s grains were decreased by 39.1%，58.1%，20.9% and 39.2% than apple replant soil，vinasse and distiller’s grains have weaker effection on inhibiting Fusarium proliferatum. In conclusion，adding vinasse and distiller’s grains in apple replant soil could change microbial community structure in soil，reduce the number of harmful fungi. Vinasse and distiller’s grains can alleviate the apple replant disease and distiller’s grains have better effect.

Key words: Malus hupeheusis, vinasse and distiller’s grains, replant disease, microorganism