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园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 809-812.

• 研究报告 • 上一篇    下一篇

新疆栽培杏群体遗传结构的SSR分析

何天明1, 2;陈学森1*;高疆生2;张大海3;徐麟3;吴燕1
  

  1. (1 山东农业大学果树生物学实验室, 山东泰安 271018; 2 塔里木大学植物科技学院, 新疆阿拉尔843300; 3 新疆农业科学院轮台国家果树资源圃, 新疆轮台841600)
  • 收稿日期:2005-07-20 修回日期:2006-04-04 出版日期:2006-08-25 发布日期:2006-08-25

Using SSR Markers to Study Popula tion Genetic Structure of Cultivated Apricots Native to Xinjiang

He Tianming1, 2;Chen Xuesen1*;Gao Jiangsheng2;Zhang Dahai3;Xu Lin3;Wu Yan1   

  1. (1Biological Lab of Pomology, Shandong Agricultural University, Tai'an, Shandong 271018, China; 2College of Plant Science and Technology, Tarim University, Alar, Xinjiang 843300, China; 3Luntai National Pomology Germplasm Garden, Academy of Agricultural Science of Xinjiang, Luntai, Xinjiang 841600, China)
  • Received:2005-07-20 Revised:2006-04-04 Online:2006-08-25 Published:2006-08-25

摘要: 利用11对SSR引物对喀什、和田和库车3个新疆栽培杏( Prunus armeniaca L. ) 品种亚群进
行了分子系统学研究。结果表明: 各位点平均期望杂合度(He = 0.2364) 显示新疆栽培杏群体具有较高的遗传多样性水平。根据基因分化系数(Gst = 0.1508) 值, 新疆杏各品种亚群的遗传分化主要存在于亚群(84.9%) 内。基于Gst测得的基因流Nm 为2.3666, 人为通过种子引种可能是其基因交流的主要方式。根据果实形态和地理起源对新疆杏进行传统分类并不能完全反映出新疆杏品种间的亲缘关系。

关键词: 杏, SSR, 遗传多样性, 群体遗传学, 聚类分析

Abstract: Eleven Prunus simple sequence repeat ( SSR) primer pairs have been used in the molecular characterization of 30 apricot (Prunus armeniaca L. ) varieties from south Xinjiang Uygur Autonomous Region, China. A higher level of genetic diversity was revealed by Neips gene diversity (He = 012364). The Gst (Gene
Divergence) of 0.1508 indicated that genetic variation existed mainly within sub-group s (84.9%). The modest amount of gene flow (Nm =2.3666) based on Gst was discovered. Itwas p roposed that artificial introduction by seeds was probably the main way of gene flow. Consequently, aditional morphological and geographical classification can not completely reflect the genetic relationship between ap ricot varieties native to Xinjiang.

Key words: Apricot, SSR, Genetic diversity, Population genetics, Clustering analysis