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园艺学报 ›› 2006, Vol. 33 ›› Issue (2): 381-384.

• 研究简报 • 上一篇    下一篇

变叶海棠遗传多样性的AFLP分析

石胜友1;成明昊2;梁国鲁2*;郭启高2;李晓林2;周志钦2*   

  1. (1 湖北省宜昌市林业局, 宜昌443000; 2 西南大学园艺园林学院, 重庆市果树学重点实验室, 重庆400716)
  • 收稿日期:2005-04-18 修回日期:2005-08-22 出版日期:2006-04-25 发布日期:2006-04-25

Genetic Diversity of Malus toringoides ( Rehd. ) Hughes Based on AFLP

Shi Shengyou1;Cheng Minghao2; Liang Guolu2*;Guo Qigao2;Li Xiaolin2;Zhou Zhiqin2*
  

  1. (1 State Forestry Administration of Yichang, Yichang 443000, China; 2College of Horticulture and Landscape, Southwest University, the Key Laboratory for Pomology of Chongqing, Chongqing 400716, China)
  • Received:2005-04-18 Revised:2005-08-22 Online:2006-04-25 Published:2006-04-25

摘要: 用8对引物对变叶海棠30个不同的变异类型进行了AFLP分析, 共扩增出656条带, 其中多态性带为568条, 平均每对引物扩增出71条多态性带, 多态性带百分率为86.59% , 各变异类型多态性比例介于0.3582~0.6296之间; 依据简单匹配系数对AFLP扩增结果进行UPGMA聚类, 30个变异类型在0.68水平上分为12个类群; 该试验从DNA水平上探明了变叶海棠的遗传多样性是变叶海棠与陇东海棠和花叶海棠产生渗入杂交形成的。

关键词: 变叶海棠, AFLP, 遗传多样性, 聚类分析

Abstract: The study ismainly on the genetic diversity of Malus toringoides (Rehd. ) Hughes by app ying AFLP. 658 bandswere obtained with 8 pairs of AFLP primers based on 30 variant types, and among them 568 (86.59% ) were polymorphic with an average of 71 bands for perAFLP p rimer, the ratio of polymorphic rate varied from 0.3582 to 0.6296. UPGMA cluster analysis based on simp le matching coefficient (SM) divided the thirty variant types into twelve group s at 0.68 level. At DNA level, the results showed that the rich diversity inM. toringoides (Rehd. ) Hugheswas derived from the introgression of M. toringoides (Rehd. )
Hughes with M. transitoria Schneid. and M. kansuensis Rehd.

Key words: Malus toringoides, AFLP, Genetic diversity, Cluster analysis