‘凤丹白’牡丹核型分析与减数分裂的细胞遗传学观察

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 411-416.

‘凤丹白’牡丹核型分析与减数分裂的细胞遗传学观察

李子峰^1,2;王　佳^2,3;胡永红^2*;刘庆华¹; 秦　俊²

(¹莱阳农学院环境艺术学院, 山东青岛266109; ²上海植物园, 上海200231; ³北京林业大学园林学院, 北京100083)

收稿日期:2006-10-30 修回日期:2007-03-19 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 胡永红

Karyotype Analysis and Cytology Observation on Meiosis of Paeonia ostii‘Phoenix White’

LI Zi-feng^1,2,WANG Jia^2,3,HU Yong-hong^2*, LIU Qing-hua¹, and QIN Jun²

(¹College of Landscape A rchitecture and Art, Laiyang Agricultural University, Qingdao, Shandong 266109, China; ²Shanghai Botanical Garden, Shanghai 200231, China; ³College of Landscape, Beijing Forestry University, Beijing 100083, China)

Received:2006-10-30 Revised:2007-03-19 Online:2007-04-25 Published:2007-04-25
Contact: HU Yong-hong

摘要/Abstract

摘要： 对江南牡丹品种‘凤丹白’的核型和花粉母细胞减数分裂行为进行研究。结果表明: ‘凤丹
白’为二倍体, 染色体数2n=10, 核型公式: 2n=2x=10=6m+2sm+2st, 属于2A型, 没有观察到随体。推测‘凤丹白’和杨山牡丹不是同物异名, 主要表现在随体的有无。与杨山牡丹的核型进行比较发现: 从杨山牡丹演化到‘凤丹’系列是由纯合到不同等级的杂合。‘凤丹白’减数分裂过程中具有不同步性, 在后期Ⅰ、末期Ⅰ观察到染色体桥, 后期Ⅱ、末期Ⅱ分别观察到“对角线”桥、不均等分裂、双桥等现象。上海地区观察‘凤丹白’花粉母细胞减数分裂的最佳取材时间为2月底～3月初。

关键词: 牡丹, 染色体, 核型, 花粉母细胞, 减数分裂

Abstract: The karyotype and meiosis course of pollen mother cell on Paeonia ostii‘PhoenixWhite’of cultivar group of tree peony from South Yangtse River of China have been observed and researched. Results show that the chromosome number of Paeonia ostii‘PhoenixWhite’is 10. It is liploid. Kitagawa is 2n=2x=10=6m + 2sm+2 st, which is belong to 2A type. It has not observed chromosome satellites. Extrapolated Paeonia ostii‘PhoenixWhite’with Paeonia ostii is not the different same thing, whether there ismainly disp lays in chromosome satellites. Compare with the karyotype of Paeon ia ostii and show that from Paeonia ostii to Paeonia ostii‘PhoenixWhite’evolved the course from pure to mixed and shut different grade. Meiosis is out of step. It was observed that the chromosome bridge was in anaphase Ⅰ, telophase Ⅰ and the‘diagonal’bridge, splits unequally, one pair of bridges, etc. were in anaphaseⅡ, telophaseⅡ. The unusual phenomenon in meiosis course of pollen mother cell of Paeonia ostii‘PhoenixWhite’have been analyzed from cell science of heredity level. The best time to observation of the pollen master cellmeiosis is end of February to the beginning of March in Shanghai.

Key words: Paeonia ostii, Chromosome, Karyotype, Pollen mother cell, Meiosis

中图分类号:

S 685.11

李子峰;王　佳;胡永红;刘庆华;秦　俊. ‘凤丹白’牡丹核型分析与减数分裂的细胞遗传学观察[J]. 园艺学报, 2007, 34(2): 411-416.

LI Zi-feng;WANG Jia;HU Yong-hong;LIU Qing-hua;and QIN Jun. Karyotype Analysis and Cytology Observation on Meiosis of Paeonia ostii‘Phoenix White’[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 411-416.

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