园艺学报 ›› 2020, Vol. 47 ›› Issue (6): 1059-1071.doi: 10.16420/j.issn.0513-353x.2019-0915

• 研究论文 • 上一篇    下一篇


黄艺烁*,谢学文*,石延霞,柴阿丽,李 磊**,李宝聚**   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2020-06-25 发布日期:2020-06-25
  • 基金资助:

Biocontrol Effect of Paenibacillus polymyxa Strain ZF197 Against Base Stem Rot of Chinese Cabbage

HUANG Yishuo*,XIE Xuewen*,SHI Yanxia,CHAI Ali,LI Lei**,and LI Baoju**   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2020-06-25 Published:2020-06-25

摘要: 从露地大白菜根际土壤中分离获得一株对白菜茎基腐病病原菌白菜立枯丝核菌(Rhizoctonia solani)有显著抑制效果的生防细菌ZF197,通过形态特征、生理生化特征和多基因系统进化树分析对其进行鉴定;通过大白菜离体叶片和温室盆栽试验研究其对白菜茎基腐病防治效果,并鉴定其在白菜根部的定殖能力;通过酶学试验和抑菌谱试验研究其生物学特性;采用三明治法测定其发酵液的最适培养基和发酵时间。结果表明,菌株ZF197为多粘类芽胞杆菌(Paenibacillus polymyxa),对大白菜离体叶片立枯丝核菌的防效可达82.35%,盆栽防效可达78.57%;该菌株可在白菜根部稳定定殖,处理20 d后定殖量趋于稳定,保持在2.37 × 105 cfu ? g-1左右;菌株ZF197具有广谱拮抗作用,能够有效抑制8种病原真菌和6种病原细菌的生长,其代谢过程中有蛋白酶和纤维素酶的产生。菌株ZF197 96 h发酵液对立枯丝核菌的抑制率最高,可达58.58%;以棉籽饼粉作为碳源的发酵液对立枯丝核菌的抑制率最高。

关键词: 大白菜, 多粘类芽胞杆菌, 立枯丝核菌, 抑菌谱, 发酵, 防效

Abstract: In this study,a biocontrol bacterium ZF197 isolated from the Chinese cabbage rhizosphere soil samples had significant inhibitory effect on Rhizoctonia solani. The strain was identified based on biological characteristics,physiological and biochemical characteristics,and multilocus sequence analysis. To investigate the control potential of strain ZF197 on R. solani,the control effect of strain ZF197 on R. solani was studied via leaves in vitro and potted plants in greenhouse inoculation experiment,and colonization abilities of strain ZF197 on the root of Chinese cabbage was via root colonization experiment. The biological characters of strain ZF197 was studied by enzymatic test and antimicrobial spectrum tests. The antagonistic ability of ZF197 was tested by the sandwich method,and the optimum culture and fermentation time of ZF197 fermentation broth were determined. The results indicated that the strain ZF197 was identified as Paenibacillus polymyxa. The control efficacy of leaves in vitro and potted plants in greenhouse was 82.35% and 78.57%,respectively. In addition,the strain could colonize the root of Chinese cabbage in a stable way,and the colonizing amount tended to be stable at about 2.37 × 105 cfu ? g-1 after the 20 d treatment. The strain ZF197 could produce protease and cellulase during the metabolic process,and exhibited broad inhibitory spectrum against eight pathogenic fungi and six pathogenic bacteria. The results of fermentation experiments indicated that the best fermentation time of ZF197 strain was at 96 h and the inhibition rate was 58.58%. Meanwhile,the fermentation broth of cottonseed meal had the best inhibitory effect on R. solani.

Key words: Chinese cabbage, Paenibacillus polymyxa, Rhizoctonia solani, antimicrobial spectrum, fermentation, control efficacy