园艺学报 ›› 2016, Vol. 43 ›› Issue (6): 1099-1106.doi: 10.16420/j.issn.0513-353x.2016-0323

• 蔬菜 • 上一篇    下一篇



  1. (农业部园艺作物生物学与种质创制重点实验室,中国农业科学院蔬菜花卉研究所,北京 100081)
  • 出版日期:2016-06-25 发布日期:2016-06-25

Function Analysis of Carrot SOC1 Homologues Responding to Photoperiod

LIU Li-jie,OU Cheng-gang,ZHAO Zhi-wei,SUN Ting-ting,and ZHUANG Fei-yun*   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Beijing 100081,China)
  • Online:2016-06-25 Published:2016-06-25


基于先期抽薹敏感的松滋野生和耐抽薹的Amsterdam胡萝卜转录组测序结果,分析胡萝卜中SOC1同源基因对光周期的响应规律及其功能。胡萝卜中存在2SOC1同源基因:DcSOC1-1DcSOC1-2,具有完整的开放阅读框(ORF),分别编码217211个氨基酸,均包含MADS-boxK-box结构域,C末端含有保守的SOC1 motif基序。系统进化树分析表明,DcSOC1-1DcSOC1-2与猕猴桃AcSOC1f和葡萄VvSOC1亲缘关系较近,属于SOC1/TM3亚家族。在长/短日照处理下,DcSOC1-1松滋野生叶片中的相对表达量均显著高于Amsterdam,且长日照能促进其表达,而DcSOC1-2松滋野生Amsterdam中的表达水平较低。在春秋两季生长过程中,DcSOC1-1松滋野生叶片中的最高表达量分别达到522.4401.7而在Amsterdam中均呈低水平表达,与先期抽薹率呈极显著正相关,说明DcSOC1-1对胡萝卜的先期抽薹起着重要作用。

关键词: 胡萝卜, 先期抽薹, 光周期, DcSOC1, qPCR


Basis on the RNA-seq analysisthe relative expression and function of carrot SUPPRESSOR OF OVEREXPRESSION OF CONSTANTS 1SOC1homologues under photoperiod treatment were investigated with wild sensitive speciesSongziand cultivarAmsterdamwhich was tolerant to premature bolting. There were two carrot SOC1 homologuesDcSOC1-1 and DcSOC1-2which were annotated with complete open reading framesORFencoding 217 and 211 amino acids respectivelywhich both containing MADS-box and K-box domain and a conserved SOC1 motif at the carboxy terminus. Phylogenetic analysis showed that DcSOC1-1 and DcSOC1-2 were closely related with kiwifruit AcSOC1f and grape VvSOC1belonging to SOC1/TM3 subfamily. The expression of DcSOC1-1 in the leaves ofSongziwas significantly higher than that inAmsterdamunder short day and long day treatmentsand long day can promote its expressionbut the expression of DcSOC1-2 inSongziandAmsterdamwas low. During the growth of spring and autumnthe maximum expression values of DcSOC1-1 inSongzileaves were 522.4 and 401.7 respectivelybut its expression was very low in levels ofAmsterdam. The correlation analysis revealed that the expression of DcSOC1-1 was significantly correlated with the ratio of premature boltingwhich indicated DcSOC1-1 played an important role in carrot premature bolting.

Key words: carrot, premature bolting, photoperiod, DcSOC1, qPCR