关键词: 胡萝卜, 先期抽薹, 光周期, DcSOC1, qPCR

Abstract:

Basis on the RNA-seq analysis，the relative expression and function of carrot SUPPRESSOR OF OVEREXPRESSION OF CONSTANTS 1（SOC1）homologues under photoperiod treatment were investigated with wild sensitive species‘Songzi’and cultivar‘Amsterdam’which was tolerant to premature bolting. There were two carrot SOC1 homologues：DcSOC1-1 and DcSOC1-2，which were annotated with complete open reading frames（ORF）encoding 217 and 211 amino acids respectively，which both containing MADS-box and K-box domain and a conserved SOC1 motif at the carboxy terminus. Phylogenetic analysis showed that DcSOC1-1 and DcSOC1-2 were closely related with kiwifruit AcSOC1f and grape VvSOC1，belonging to SOC1/TM3 subfamily. The expression of DcSOC1-1 in the leaves of‘Songzi’was significantly higher than that in‘Amsterdam’under short day and long day treatments，and long day can promote its expression，but the expression of DcSOC1-2 in‘Songzi’and‘Amsterdam’was low. During the growth of spring and autumn，the maximum expression values of DcSOC1-1 in‘Songzi’leaves were 522.4 and 401.7 respectively，but its expression was very low in levels of‘Amsterdam’. The correlation analysis revealed that the expression of DcSOC1-1 was significantly correlated with the ratio of premature bolting，which indicated DcSOC1-1 played an important role in carrot premature bolting.

Key words: carrot, premature bolting, photoperiod, DcSOC1, qPCR