园艺学报 ›› 2013, Vol. 40 ›› Issue (3): 426-432.

• 果树 • 上一篇    下一篇


刘学生,陈 龙,王金鑫,李 莉,彭建营   

  1. 1 河北农业大学园艺学院,河北保定 071001;2 河北省农林科学院昌黎果树研究所,河北昌黎 066600
  • 出版日期:2013-03-25 发布日期:2013-03-25

Discovery and Identification of Natural Triploid Ploidy of Chinese Jujube

 LIU  Xue-Sheng, CHEN   Long, WANG  Jin-Xin, LI   Li, PENG  Jian-Ying   

  1. (1 College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China;2 Fruit Institute,Hebei
    Academy of Agriculture and Forestry Sciences,Changli,Hebei 066600,China)
  • Online:2013-03-25 Published:2013-03-25

摘要: 采用酶解去壁低渗法对枣品种‘苹果枣’进行了核型分析,其核型公式为:2n = 3x = 36 =
21m + 15sm(2SAT),核型为2A 型,是迄今为止新发现的第2 个栆自然三倍体品种。对花粉母细胞减数
最少含有7 个三价体,最多则含10 个三价体,3 个染色体组之间具有很高的同源性,后期Ⅰ和后期Ⅱ出

关键词: 枣, 自然三倍体, 核型, 花粉母细胞, 减数分裂

Abstract: The methods of eliminating walls by enzymolysis and low osmosis were used to analyze
the chromosome karyotype of Ziziphus jujuba Mill.‘Pingguozao’. The results showed that the karyotype
formula was 2n = 3x = 36 = 21m + 15sm(2SAT),and the karyotype was 2A type. It was second of the
newly discovered natural triploid cultivar. The meiosis course of pollen mother cells of‘Pingguozao’was
observed,and the various periods of the meiosis were described and analyzed. The results showed that the
meiosis of ‘Pingguozao’was irregular. The diakinesis appeared univalent,bivalent and trivalent,and 57.5%
of them were trivalent,at least 7 trivalents,at most contained 10 trivalents. The three genomes had very
high homology. The lagging chromosomes and chromosome unequal separation phenomenon were
observed in anaphase I and II. Some cells also produced few triad and pentad in telophaseⅡ,and formed
unequal spores,and led to highly gametophyte sterility. In view of the above it was deduced that
‘Pingguozao’was autotriploid. The RAPD fingerprints of‘Pingguozao’and‘Zanhuangdazao’were
different,which were two different natural triploid cultivars.

Key words: Ziziphus jujuba Mill., natural triploid, karyotype, pollen mother cell, meiosis